PELSA-Decipher: a software tool for the processing and interpretation of ligand-protein interaction dataset acquired by PELSA

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Abstract Knowledge of protein-ligand interactions is essential for comprehending various aspects of life science, including drug mechanisms of action, regulatory processes in cellular metabolism and signaling, etc. Recently, a robust ligand modification-free method, termed as peptide-centric local stability assay (PELSA), is developed to identify the protein targets and binding regions of diverse ligands at proteomics scale. This method has unprecedented sensitivity and can be broadly applied to ligands including drugs, metabolites, metal ions, antibodies tec. However, the extraction of key information on ligand-protein interaction, including the binding protein, binding site, and binding affinity, is not a trivial task, which hampers the wide utilization of PELSA strategy by the research community. To address this, we developed a software tool, PELSA-Decipher, to facilitate the efficient processing of dataset obtained in PELSA experiment, including raw data processing, result visualization, and the generation of reports and high-quality images, thereby will greatly promote the broader applications of PELSA strategy. The PELSA-Decipher software can be downloaded free of charge from the website https://github.com/DICP-1809 .

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