Untargeted Metabolomic Profiling of Colonic Mucosa in Individuals with Irritable Bowel Syndrome
Rattachement africain : pl, dk. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Background: Irritable Bowel Syndrome (IBS) is a complex disorder characterized by altered gut–brain interactions, with gastrointestinal microbiota and metabolic dysregulation playing key roles in its pathophysiology. Identifying specific metabolic alterations within the colonic mucosa may enhance our understanding of IBS and contribute to improved diagnostic and therapeutic approaches. Methods: This cross-sectional study analyzed the metabolomic profiles of colonic mucosal biopsies from 44 IBS patients assessed with ROME IV criteria and 69 healthy controls undergoing colonoscopy. Untargeted metabolomic profiling was conducted using liquid chromatography–mass spectrometry (LC-MS), and differential metabolite analysis was performed via fold-change calculations and machine learning-based classification. Results: IBS patients exhibited distinct mucosal metabolic profiles, with significantly elevated levels of N-acetylneuraminic acid and 1-palmitoylglycerol, suggesting compromised epithelial integrity and increased gut permeability. In contrast, cis-4-hydroxycyclohexanecarboxylic acid, a metabolite associated with protective mucosal functions, was reduced. Random Forest analysis identified these metabolites as key discriminatory features between IBS and control groups, reinforcing their potential role as biomarkers for IBS-related mucosal alterations. Conclusions: Our study highlights the unique metabolomic signatures of IBS at the mucosal level, emphasizing the role of microbial metabolites in disease pathology. These findings may facilitate the development of novel diagnostic tools and targeted therapeutic strategies, advancing personalized management for IBS patients.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Untargeted Metabolomic Profiling of Colonic Mucosa in Individuals with Irritable Bowel Syndrome
- Date Crossref
- 05/03/2025
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Pomeranian Medical University Department of Gastroenterology pays non établi dans la noticeUniversité ou école supérieure
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Polbud-Pomorze (Poland) pays non établi dans la noticeEntreprise
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University of Southern Denmark Department of Clinical Research pays non établi dans la noticeUniversité ou école supérieure
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Odense University Hospital Research Unit pays non établi dans la noticeOrganisme public
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Svendborg Sygehus pays non établi dans la noticeÉtablissement de santé
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Sanprobi sp. z o.o. sp.k. pays non établi dans la noticeInstitution
Department of Gastroenterology — Pomeranian Medical University, Polbud-Pomorze (Poland) et Department of Clinical Research — University of Southern Denmark, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.