Integrative analyses reveal Bna‐miR397a–BnaLAC2 as a potential modulator of low‐temperature adaptability in Brassica napus L.
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Le résumé fourni par la source
Brassica napus L. (B. napus) is a major edible oil crop grown around the southern part of China, which often faces cold stress, posing potential damage to vegetative tissues. To sustain growth and reproduction, a detailed understanding of fundamental regulatory processes in B. napus against long-term low temperature (LT) stress is necessary for breeders to adjust the level of LT adaption in a given region and is therefore of great economic importance. Till now, studies on microRNAs (miRNAs) in coping with LT adaption in B. napus are limited. Here, we performed an in-depth analysis on two B. napus varieties with distinct adaptability to LT stress. Through integration of RNA sequencing (RNA-seq) and small RNA-sequencing (sRNA-seq), we identified 106 modules comprising differentially expressed miRNAs and corresponding potential targets based on strong negative correlations between their dynamic expression patterns. Specifically, we demonstrated that Bna-miR397a post-transcriptionally regulates a LACCASE (LAC) gene, BnaLAC2, to enhance the adaption to LT stresses in B. napus by reducing the total lignin remodelling and ROS homeostasis. In addition, the miR397-LAC2 module was also proved to improve freezing tolerance of Arabidopsis, indicating a conserved role of miR397-LAC2 in Cruciferae plants. Overall, this work provides the first description of a miRNA-mediated-module signature for LT adaption and highlights the prominent role of laccase in future breeding programme of LT tolerant B. napus.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Integrative analyses reveal <i>Bna‐miR397a–BnaLAC2</i> as a potential modulator of low‐temperature adaptability in <i>Brassica napus</i> L.
- Date Crossref
- 04/03/2025
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Chinese Academy of Agricultural Sciences pays non établi dans la noticeOrganisme public
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Oil Crops Research Institute pays non établi dans la noticeStructure de recherche
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Yangtze University pays non établi dans la noticeUniversité ou école supérieure
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State Key Laboratory of Biocatalysis and Enzyme Engineering pays non établi dans la noticeStructure de recherche
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Hubei University State Key Laboratory of Biocatalysis and Enzyme Engineering pays non établi dans la noticeUniversité ou école supérieure
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Hubei Academy of Agricultural Sciences pays non établi dans la noticeOrganisme public
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Yangzhou University Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology pays non établi dans la noticeUniversité ou école supérieure
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Key Laboratory of Biology and Genetic Improvement of Oil Crops pays non établi dans la noticeStructure de recherche
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These authors contributed equally to this work pays non établi dans la noticeInstitution
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School of Life Sciences State Key Laboratory of Biocatalysis and Enzyme Engineering pays non établi dans la noticeUniversité ou école supérieure
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Food Crops Institute pays non établi dans la noticeStructure de recherche
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College of Agriculture Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology pays non établi dans la noticeUniversité ou école supérieure
Chinese Academy of Agricultural Sciences, Oil Crops Research Institute et Yangtze University, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.