In vitro development of a radicular cyst: A morphological investigation of a spheroid cyst-like model associated with fibroblasts
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Le résumé fourni par la source
OBJECTIVE: The aim of this study was to improve the in vitro model of tooth radicular cyst previously developed by incorporating stromal components and to describe histologic analysis. DESIGN: hFIB) to mimic stromal microenvironment. Micrographs were taken to evaluate the cystic stability and dispersion area, while histological hematoxylin/eosin staining was used to measure the ratio of epithelial lining area. Analysis was conducted on days 1, 3, and 7 using ImageJ software. Statistical analyses were performed using GraphPad Prism 5 software. RESULTS: The model, with fibroblasts included, preserved the cystic structure and allowed cyst growth, with an increase in both the area and dispersion of the cystic structure throughout the experimental period (p < 0.05). Histological analysis of the cyst model revealed morphological similarities with in vivo tooth radicular cyst biopsies, showing a cystic cavity lined by an epithelial layer, surrounded by collagen and fibroblasts. Additionally, the cavity area increased while the limiting epithelial area decreased. The highest epithelial area-to-total area ratio was observed in day 1 spheroids, while the lowest was found on day 7 (p < 0.05). CONCLUSION: The incorporation of fibroblasts improved the in vitro cystogenesis model, since it did not interfere with the model's development and more closely mimicked the in vivo microenvironment.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- In vitro development of a radicular cyst: A morphological investigation of a spheroid cyst-like model associated with fibroblasts
- Date Crossref
- 01/04/2025
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Universidade Federal do Rio Grande do Sul pays non établi dans la noticeUniversité ou école supérieure
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Morpho (United States) pays non établi dans la noticeEntreprise
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Faculdades Guarulhos pays non établi dans la noticeInstitution
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Basic Research Center pays non établi dans la noticeStructure de recherche
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Department of Morphological Sciences pays non établi dans la noticeInstitution
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Pos Graduate Program pays non établi dans la noticeInstitution
Universidade Federal do Rio Grande do Sul, Morpho (United States) et Faculdades Guarulhos, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.