One-step bioprinting of endothelialized, self-supporting arterial and venous networks
Rattachement africain : us, dk, in, kr. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Advances in biofabrication have enabled the generation of freeform perfusable networks mimicking vasculature. However, key challenges remain in the effective endothelialization of these complex, vascular-like networks, including cell uniformity, seeding efficiency, and the ability to pattern multiple cell types. To overcome these challenges, we present an integrated fabrication and endothelialization strategy to directly generate branched, endothelial cell-lined networks using a diffusion-based, embedded 3D bioprinting process. In this strategy, a gelatin microparticle sacrificial ink delivering both cells and crosslinkers is extruded into a crosslinkable gel precursor support bath. A self-supporting, perfusable structure is formed by diffusion-induced crosslinking, after which the sacrificial ink is melted to allow cell release and adhesion to the printed lumen. This approach produces a uniform cell lining throughout networks with complex branching geometries, which are challenging to uniformly and efficiently endothelialize using conventional perfusion-based approaches. Furthermore, the biofabrication process enables high cell viability (>90%) and the formation of a confluent endothelial layer providing vascular-mimetic barrier function and shear stress response. Leveraging this strategy, we demonstrate for the first time the patterning of multiple endothelial cell types, including arterial and venous cells, within a single arterial-venous-like network. Altogether, this strategy enables the fabrication of multi-cellular engineered vasculature with enhanced geometric complexity and phenotypic heterogeneity.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- One-step bioprinting of endothelialized, self-supporting arterial and venous networks
- Date Crossref
- 11/02/2025
- Éditeur
- IOP Publishing
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Stanford University Department of Materials Science and Engineering pays non établi dans la noticeUniversité ou école supérieure
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Technical University of Denmark Department of Health Technology pays non établi dans la noticeUniversité ou école supérieure
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California Institute for Regenerative Medicine pays non établi dans la noticeOrganisme public
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Institute for Stem Cell Biology and Regenerative Medicine pays non établi dans la noticeStructure de recherche
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University of Washington Department of Pediatrics pays non établi dans la noticeUniversité ou école supérieure
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Center for Cancer and Blood Disorders pays non établi dans la noticeStructure de recherche
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Seoul National University Department of Agriculture pays non établi dans la noticeUniversité ou école supérieure
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Seattle Children's Research Institute Ben Towne Center for Childhood Cancer and Blood Disorders Research pays non établi dans la noticeStructure de recherche
Department of Materials Science and Engineering — Stanford University, Department of Health Technology — Technical University of Denmark et California Institute for Regenerative Medicine, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.