Polystyrene nanoparticles induce DNA damage and apoptosis in HeLa cells
Rattachement africain : it. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Nanoplastics (NPs) are plastic particles, typically less than 100 nm in size, that result from daily life products as well as the degradation of larger plastic debris. Due to their small size and chemical composition, they can interact with biological systems in ways that larger plastic particles cannot. Humans are continuously exposed to NPs and several studies showed the potentially toxic effects of these latter on health. Polystyrene nanoplastics (PS-NPs) are the prevalent form of nanoparticles found in the environment and their cellular uptake can cause cytotoxicity and structural alteration of biomolecules. Thus, there is an urgent need for evaluation of the genotoxic effects of PS-NPs on human cell models. Through different and complementary experimental approaches, we investigated the potential genotoxic and cytotoxic effects of PS-NPs exposure on HeLa cell lines. We highlighted the genotoxic effects of polystyrene nanoplastics by showing the formation of multinuclei and micronuclei in all the studied concentrations and time points, also at short incubation time (6 h) and low concentration. At higher concentrations, we demonstrate the presence of apoptotic and necrotic cells outlining the acute cytotoxic effects of nanoplastics. The genotoxic potential is further highlighted by the presence of low molecular weight DNA fragments in PS-NPs treated cells, and by the relationship between polystyrene nanoplastics and γ-H2AX. Thus, our data provide important insights at a cellular level into the possible risks produced by these nanoparticles and recommend further deeper research studies to address the impacts of nanoplastics on human health.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Polystyrene nanoparticles induce DNA damage and apoptosis in HeLa cells
- Date Crossref
- 01/01/2025
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Federico II University Hospital pays non établi dans la noticeÉtablissement de santé
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University of Naples Federico II pays non établi dans la noticeUniversité ou école supérieure
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University of Campania "Luigi Vanvitelli" Department of Environmental pays non établi dans la noticeUniversité ou école supérieure
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University of Foggia Department of Clinical and Experimental Medicine pays non établi dans la noticeUniversité ou école supérieure
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Institute of Crystallography pays non établi dans la noticeStructure de recherche
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Institute of Polymers pays non établi dans la noticeStructure de recherche
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University of Naples "Federico II" Naples Department of Biology pays non établi dans la noticeUniversité ou école supérieure
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Institute for Polymers pays non établi dans la noticeStructure de recherche
Federico II University Hospital, University of Naples Federico II et Department of Environmental — University of Campania "Luigi Vanvitelli", avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.