Altered exosomal miRNA profiles in patients with paraneoplastic cerebellar degeneration
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Le résumé fourni par la source
OBJECTIVE: Patients with ovarian cancer (OC) may develop anti-Yo-associated paraneoplastic cerebellar degeneration (PCD)-a cerebellar ataxia associated with tumor-induced autoimmunity against CDR2 and CDR2L proteins. Dysregulation of circulating exosomal microRNAs (miRNAs) occur in OC. Here, we investigated whether PCD is associated with changes in the exosomal miRNA profiles of OC patients. METHODS: Serum exosomes were isolated from patients with OC (n = 15), patients with OC and anti-Yo-associated PCD (n = 14) and healthy controls (HC, n = 15). Small RNA sequencing was used to identify differentially expressed miRNAs. Receiver operating characteristic curves were used to evaluate biomarker sensitivity and specificity, and miRNA target prediction analysis was employed to elucidate gene targets. RESULTS: OC patients with PCD exhibited a distinct exosomal miRNA expression profile. We detected 103 differentially expressed exosomal miRNAs in PCD patients compared to OC patients without PCD and 139 differentially expressed exosomal miRNAs compared to controls. Particularly miR-486-5p, miR-4732-5p, miR-98-5p and miR-21-5p exhibited notable sensitivity and specificity for discriminating PCD patients from both OC patients without PCD and healthy controls. miRNA target prediction showed that several of the differentially expressed miRNAs in PCD patients targeted the CDR2 and CDR2L genes. INTERPRETATION: Our results demonstrate that OC patients with anti-Yo-associated PCD exhibit a distinct exosomal miRNA profile compared to OC patients without PCD. Several of the differentially expressed exosomal miRNAs in PCD patients showed diagnostic potential and may hold relevance for understanding the pathogenesis of PCD.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Altered exosomal <scp>miRNA</scp> profiles in patients with paraneoplastic cerebellar degeneration
- Date Crossref
- 29/10/2024
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Bergen Department of Clinical Medicine pays non établi dans la noticeUniversité ou école supérieure
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Norwegian Institute of Public Health Department of Health Registry Research and Development pays non établi dans la noticeOrganisme public
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Haukeland University Hospital Department of Obstetrics and Gynaecology pays non établi dans la noticeÉtablissement de santé
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Lyon 1 Université pays non établi dans la noticeUniversité ou école supérieure
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Centre National de la Recherche Scientifique pays non établi dans la noticeOrganisme public
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Inserm pays non établi dans la noticeOrganisme public
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Hospices Civils de Lyon pays non établi dans la noticeÉtablissement de santé
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Departments of Neurology and Clinical Medicine Neuro‐SysMed ‐ Centre of Excellence for Experimental Therapy in Neurology Bergen Norway pays non établi dans la noticeÉtablissement de santé
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Université de Lyon Université Claude Bernard Lyon 1 Lyon France MeLiS ‐ UCBL ‐ CNRS UMR 5284 ‐ INSERM U1314 pays non établi dans la noticeUniversité ou école supérieure
Department of Clinical Medicine — University of Bergen, Department of Health Registry Research and Development — Norwegian Institute of Public Health et Department of Obstetrics and Gynaecology — Haukeland University Hospital, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.