Integrating Bulk and Single-cell RNA-seq to Construct a Macrophage-related Prognostic Model for Prognostic Stratification in Triple-negative Breast Cancer
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Le résumé fourni par la source
Background: Triple-negative breast cancer (TNBC) is a poor prognostic subtype of breast cancer due to limited treatment.Macrophage plays a critical role in tumor growth and survival.Our study intends to explore the heterogeneity of macrophage in TNBC and establish a macrophage-related prognostic model for TNBC prognostic stratification.Materials and Methods: Seurat package was conducted to analyze the single-cell RNA expression profilers.The cell types were identified by the markers derived from public research and online database.The cell-cell interactions were calculated by the CellChat package.Monocle package was used to visualize the cell trajectory of macrophages.The prognostic model was constructed by six macrophage-related genes after a series of selections.The expression of six genes were validated in normal and TNBC tissues.And several potential agents for high-risk TNBC patients were analyzed by Connectivity Map analysis.Results: Nine cell types were identified, and the macrophages were highly enriched in TNBC samples.five distinct subgroups of macrophage were identified.Notably, SPP1+ tumor-associated macrophages exhibited a poor prognosis.The prognostic model was constructed by HSPA6, LPL, IDO1, ALDH2, TK1, and QPCT with good predictive accuracy at 3-, 5-years overall survival for TNBC patients in both training and external test cohorts.Finally, several drugs were identified for the high-risk TNBC patients decided by model.Conclusion: Our study provides a valuable source for clarifying macrophage heterogeneity in TNBC, and a promising tool for prognostic risk stratification of TNBC.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Integrating Bulk and Single-cell RNA-seq to Construct a Macrophage-related Prognostic Model for Prognostic Stratification in Triple-negative Breast Cancer
- Date Crossref
- 01/01/2024
- Éditeur
- Ivyspring International Publisher
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Tianjin Medical University Cancer Institute and Hospital Key Laboratory of Breast Cancer Prevention and Therapy pays non établi dans la noticeÉtablissement de santé
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Tianjin Medical University Department of General Surgery pays non établi dans la noticeUniversité ou école supérieure
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Tianjin Medical University General Hospital pays non établi dans la noticeÉtablissement de santé
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Key Laboratory of Cancer Prevention and Therapy pays non établi dans la noticeStructure de recherche
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Tianjin's Clinical Research Center for Cancer pays non établi dans la noticeStructure de recherche
Key Laboratory of Breast Cancer Prevention and Therapy — Tianjin Medical University Cancer Institute and Hospital, Department of General Surgery — Tianjin Medical University et Tianjin Medical University General Hospital, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.