Carbapenem-resistant Klebsiella oxytoca transmission linked to preoperative shaving in emergency neurosurgery, tracked by rapid detection via chromogenic medium and whole genome sequencing
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Le résumé fourni par la source
Objectives This study describes the detection and tracking of emergency neurosurgical cross-transmission infections with carbapenem-resistant Klebsiella oxytoca (CRKO). Methods We conducted an epidemiological investigation and a rapid screening of 66 surveillance samples using the chromogenic selective medium. Two CRKO isolates from infected patients and three from the preoperative shaving razors had similar resistance profiles identified by the clinical laboratory. Results The whole genome sequencing (WGS) results identified all isolates as Klebsiella michiganensis (a species in the K. oxytoca complex) with sequence type 29 (ST29) and carrying resistance genes blaKPC-2 and blaOXY-5, as well as IncF plasmids. The pairwise average nucleotide identity values of 5 isolates ranged from 99.993% to 99.999%. Moreover, these isolates displayed a maximum genetic difference of 3 among 5,229 targets in the core genome multilocus sequence typing scheme, and the razors were confirmed as the contamination source. After the implementation of controls and standardized shaving procedures, no new CRKO infections occurred. Conclusion Contaminated razors can be sources of neurosurgical site infections with CRKO, and standard shaving procedures need to be established. Chromogenic selective medium can help rapidly identify targeted pathogens, and WGS technologies are effective mean in tracking the transmission source in an epidemic or outbreak investigation. Our findings increase the understanding of microbial transmission in surgery to improve patient care quality.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Carbapenem-resistant Klebsiella oxytoca transmission linked to preoperative shaving in emergency neurosurgery, tracked by rapid detection via chromogenic medium and whole genome sequencing
- Date Crossref
- 17/10/2024
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Anhui Medical University Department of Hospital Infection Prevention and Control pays non établi dans la noticeUniversité ou école supérieure
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Second Affiliated Hospital of Anhui Medical University pays non établi dans la noticeÉtablissement de santé
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Fourth Affiliated Hospital of Anhui Medical University pays non établi dans la noticeÉtablissement de santé
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Jinan University pays non établi dans la noticeUniversité ou école supérieure
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Southern University of Science and Technology The First Affiliated Hospital pays non établi dans la noticeUniversité ou école supérieure
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The Second Clinical Medical College Department of Laboratory Medicine pays non établi dans la noticeUniversité ou école supérieure
Department of Hospital Infection Prevention and Control — Anhui Medical University, Second Affiliated Hospital of Anhui Medical University et Fourth Affiliated Hospital of Anhui Medical University, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.