Isolation and Antibiofilm Activity of Bacteriophages against Cutibacterium acnes from Patients with Periprosthetic Joint Infection
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Le résumé fourni par la source
Background: Infections following shoulder surgery, particularly periprosthetic joint infection (PJI), are challenging to treat. Cutibacterium acnes is the causative pathogen in 39% to 76% of these cases. This study explores the efficacy of bacteriophage therapy as an alternative to conventional antibiotics for treating such infections. Methods: Nine phages with lytic activity were isolated from the skin of humans using C. acnes ATCC 6919 as the indicator host. These phages were tested individually or in combination to assess host range and antibiofilm activity against clinical strains of C. acnes associated with PJIs. The phage cocktail was optimized for broad-spectrum activity and tested in vitro against biofilms formed on titanium discs to mimic the prosthetic environment. Results: The isolated phages displayed lytic activity against a range of C. acnes clinical isolates. The phage cocktail significantly reduced the bacterial load of C. acnes strains 183, 184, and GG2A, as compared with untreated controls (p < 0.05). Individual phages, particularly CaJIE7 and CaJIE3, also demonstrated significant reductions in bacterial load with respect to specific strains. Moreover, phages notably disrupted the biofilm structure and reduced biofilm biomass, confirming the potential of phage therapy in targeting biofilm-associated infections. Conclusions: Our preclinical findings support the potential of phage therapy as a viable adjunct to traditional antibiotics for treating C. acnes infections in orthopedic device-related infections. The ability of phages to disrupt biofilms may be particularly beneficial for managing infections associated with prosthetic implants.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Isolation and Antibiofilm Activity of Bacteriophages against Cutibacterium acnes from Patients with Periprosthetic Joint Infection
- Date Crossref
- 10/10/2024
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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AO Foundation pays non établi dans la noticeOrganisation à but non lucratif
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KU Leuven pays non établi dans la noticeUniversité ou école supérieure
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Swiss Federal Laboratories for Materials Science and Technology pays non établi dans la noticeStructure de recherche
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AO Research Institute Davos pays non établi dans la noticeStructure de recherche
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Department of Development and Regeneration pays non établi dans la noticeInstitution
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University Hospitals Leuven Department of Trauma Surgery pays non établi dans la noticeUniversité ou école supérieure
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Laboratory for Biointerfaces pays non établi dans la noticeStructure de recherche
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Laboratory of Gene Technology pays non établi dans la noticeStructure de recherche
AO Foundation, KU Leuven et Swiss Federal Laboratories for Materials Science and Technology, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.