Periodontal Ligament Reactions to Orthodontic Force: A Transcriptomic Study on Maxillary and Mandibular Human Premolars
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Le résumé fourni par la source
AIMS: Orthodontic force (OF) induces a variety of reactions in the periodontal ligament (PDL) that could potentially account for individual variability regarding orthodontic tooth movement (OTM). This study investigates the transcriptomic profile of human PDL tissue subjected to OF in vivo for 7 and 28 days, additionally comparing the differences between maxillary and mandibular PDL. METHODS: Healthy patients requiring orthodontic premolar extractions were randomly assigned to one of three groups: control (CG) where no OF was applied, 7 days and 28 days, where premolars were extracted either 7 or 28 days after the application of a 50-100 g OF. Total RNA was extracted from the PDL tissue and analyzed via RNA-seq. Differentially expressed genes (DEGs) were identified using a false discovery rate and fold change threshold of < 0.05 and ≥ 1.5 respectively. Functional and Protein-Protein Interaction analysis were performed. RESULTS: After 7 days of OF, the reaction of PDL to OF is characterized by cell responses to stress, increased bone resorption, inflammation and immune response, and decreased bone formation. In contrast, after 28 days, bone regeneration is more prominent, and processes of bone homeostasis, immune response, and cell migration are present. The response of maxillary and mandibular PDL was different. Bone resorption was observed in the maxilla at 7 and 28 days, while in the mandible expression of cell proliferation and transcriptional activity were predominant after 28 days of OF. CONCLUSIONS: The early reaction of the PDL to OF corresponds with increased bone resorption and decreased bone formation. After 28 days, bone formation became more prominent. The maxillary and mandibular PDL present asynchronous responses during OTM. These findings enhance our comprehension of the mechanisms underlying the origin-specific responses of PDL to different lengths of OF, which is potentially relevant in the development of personalized therapeutic strategies.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Periodontal Ligament Reactions to Orthodontic Force: A Transcriptomic Study on Maxillary and Mandibular Human Premolars
- Date Crossref
- 09/10/2024
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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KU Leuven Department of Oral Health Sciences‐Orthodontics pays non établi dans la noticeUniversité ou école supérieure
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University of Hong Kong Division of Periodontology and Implant Dentistry pays non établi dans la noticeUniversité ou école supérieure
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Vlaams Instituut voor Biotechnologie pays non établi dans la noticeStructure de recherche
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VIB-KU Leuven Center for Brain & Disease Research pays non établi dans la noticeStructure de recherche
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University Hospitals Leuven Department of Oral Health Sciences-Orthodontics pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Dentistry Division of Periodontology and Implant Dentistry pays non établi dans la noticeUniversité ou école supérieure
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Laboratory for Neurobiology pays non établi dans la noticeStructure de recherche
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Center for Brain and Disease Research pays non établi dans la noticeInstitution
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Laboratory of Gene Regulation and Disease pays non établi dans la noticeStructure de recherche
Department of Oral Health Sciences‐Orthodontics — KU Leuven, Division of Periodontology and Implant Dentistry — University of Hong Kong et Vlaams Instituut voor Biotechnologie, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.