Bioactive peptides from food waste: New innovative bio-nanocomplexes to enhance cellular uptake and biological effects

Le résumé fourni par la source

Mastitis is the most important bovine disease, causing dramatic economic losses to the dairy industry, worldwide. This study explores the valorization of whey from cows affected by mastitis, through a novel separation approach. Surface Active Maghemite Nanoparticles (SAMNs) were used as magnetic baits to selectively bind bioactive peptides with potential health benefits. Advanced techniques such as HPLC and LC-MS/MS highlighted SAMN capability of isolating a restricted group of peptides, drastically diverging from the control profile (Solid Phase Extraction, SPE) and characterized by a peculiar acidic residue distribution. Most importantly, both magnetically purified and nano-immobilized peptides (SAMN@peptides) showed protective activity against oxidative stress and inflammation, when tested on Caco-2 cells; with SAMN@peptides being associated with the strongest biological effect. SAMNs exhibited excellent characteristics, they are environmentally sustainable, and their synthesis is cost-effective prompting at a scalable and selective tool for capturing bioactive peptides, with potential applications in functional foods and nutraceuticals. • Milk whey from mastitis affected cow valorization by extracting bioactive peptides. • Functional bio-corona on peptide selective nanoparticles was explored. • New bioactive peptides protect human cells against oxidative stress and inflammation. • Nanoparticle-peptide hybrids show a high biological activity on human cells. • The proposed nanotechnological strategy can be used in food and health fields.

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