Identification of the tumor metastasis-related tumor subgroups overexpressed NENF in triple-negative breast cancer by single-cell transcriptomics
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Le résumé fourni par la source
Tumor metastasis is a continuous and dynamic process and is a major cause of tumor-related death in triple-negative breast cancer. However, this biological process remains largely unknown in triple-negative breast cancer. The emergence of single-cell sequencing enables a deeper understanding of the tumor microenvironment and provides a new strategy for discovering the potential mechanism of tumor metastasis. Herein, we integrated the single-cell expression profiling of primary and metastatic triple-negative breast cancer by Seurat package. Nine tumor cell subgroups were identified. Enrichment analysis suggested tumor subgroups (C0, C4) were associated with tumor metastasis with poor prognosis in TNBC. Weighted gene co-expression network was constructed and identified NENF was a metastasis-related gene. Subsequently, RT-qPCR, Immunohistochemistry, and western blot confirmed NENF is highly expressed in TNBC tissues. And cell function assays indicated NENF promote cell invasion and migration through regulating EMT in TNBC. Finally, TIDE and Connectivity Map database suggest the candidate drugs for targeting NENF. In conclusion, our findings provide a new insight into the progression and metastasis of TNBC and uncover NENF may be a prognostic biomarker and potential therapy targets.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Identification of the tumor metastasis-related tumor subgroups overexpressed NENF in triple-negative breast cancer by single-cell transcriptomics
- Date Crossref
- 18/09/2024
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Tianjin Medical University Cancer Institute and Hospital Key Laboratory of Breast Cancer Prevention and Therapy pays non établi dans la noticeÉtablissement de santé
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Tianjin Medical University General Hospital Department of General Surgery pays non établi dans la noticeÉtablissement de santé
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Tianjin Medical University pays non établi dans la noticeUniversité ou école supérieure
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Key Laboratory of Cancer Prevention and Therapy pays non établi dans la noticeStructure de recherche
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Tianjin's Clinical Research Center for Cancer pays non établi dans la noticeStructure de recherche
Key Laboratory of Breast Cancer Prevention and Therapy — Tianjin Medical University Cancer Institute and Hospital, Department of General Surgery — Tianjin Medical University General Hospital et Tianjin Medical University, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.