Rice Husk Silica Liquid Enhances Autophagy and Reduces Overactive Immune Responses via TLR-7 Signaling in Lupus-Prone Models
Rattachement africain : tw. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Systemic lupus erythematosus (SLE) is a chronic autoimmune disorder characterized by widespread inflammation and multi-organ damage. Toll-like receptor 7 (TLR-7) and autophagy have been implicated in SLE pathogenesis. Rice husk silica liquid (RHSL) has shown potential for modulating inflammatory responses, but its effects on SLE have not been thoroughly investigated. This study aims to evaluate the impact of RHSL on immune responses and autophagy in an in vitro model of SLE, focusing on its effects on TLR-7 signaling, cytokine production, and autophagy modulation. RAW264.7 cells and human peripheral blood mononuclear cells (PBMCs) from healthy donors and SLE patients were used. Cells were stimulated with LPS or TLR-7 agonists and treated with RHSL. Cell viability was assessed, and cytokine levels (TNF-α and IL-6) were measured by ELISA. Autophagy-related proteins (LC3II, ATG5-ATG12) were analyzed by Western blotting. The effect of autophagy inhibition was studied using 3-methyladenine (3-MA). RHSL did not affect cell viability but significantly reduced TNF-α production in LPS- and TLR-7 agonist-stimulated RAW264.7 cells and PBMCs. RHSL enhanced autophagy, as evidenced by increased LC3II and ATG5-ATG12 conjugation in both RAW264.7 cells and SLE patient-derived PBMCs. The reduction in TNF-α production by RHSL was attenuated by 3-MA, indicating that autophagy plays a role in this process. RHSL also inhibited the translocation of phosphorylated NF-κB into the nucleus, suggesting a mechanism for its anti-inflammatory effects. RHSL exhibits potential as an immunomodulatory agent in SLE by enhancing autophagy and modulating TLR-7 signaling pathways. These findings suggest that RHSL could offer therapeutic benefits for managing inflammatory responses in SLE and warrant further investigation into its clinical applications.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Rice Husk Silica Liquid Enhances Autophagy and Reduces Overactive Immune Responses via TLR-7 Signaling in Lupus-Prone Models
- Date Crossref
- 27/08/2024
- Éditeur
- MDPI AG
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
I-Shou University Department of Internal Medicine pays non établi dans la noticeUniversité ou école supérieure
-
E-Da Hospital pays non établi dans la noticeÉtablissement de santé
-
National Taiwan Normal University pays non établi dans la noticeUniversité ou école supérieure
-
National Kaohsiung Marine University pays non établi dans la noticeUniversité ou école supérieure
-
National Kaohsiung University of Science and Technology Department of Seafood Science pays non établi dans la noticeUniversité ou école supérieure
-
College of Medical Science and Technology Department of Medical Laboratory Science pays non établi dans la noticeUniversité ou école supérieure
-
School of Medicine pays non établi dans la noticeUniversité ou école supérieure
-
School of Life Science Graduate Programs of Nutrition Science pays non établi dans la noticeUniversité ou école supérieure
-
E-Da Cancer Hospital Department of Internal Medicine pays non établi dans la noticeÉtablissement de santé
Department of Internal Medicine — I-Shou University, E-Da Hospital et National Taiwan Normal University, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.