Design of intrinsically disordered protein variants with diverse structural properties
Rattachement africain : dk, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Intrinsically disordered proteins (IDPs) perform a broad range of functions in biology, suggesting that the ability to design IDPs could help expand the repertoire of proteins with novel functions. Computational design of IDPs with specific conformational properties has, however, been difficult because of their substantial dynamics and structural complexity. We describe a general algorithm for designing IDPs with specific structural properties. We demonstrate the power of the algorithm by generating variants of naturally occurring IDPs that differ in compaction, long-range contacts, and propensity to phase separate. We experimentally tested and validated our designs and analyzed the sequence features that determine conformations. We show how our results are captured by a machine learning model, enabling us to speed up the algorithm. Our work expands the toolbox for computational protein design and will facilitate the design of proteins whose functions exploit the many properties afforded by protein disorder.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Design of intrinsically disordered protein variants with diverse structural properties
- Date Crossref
- 30/08/2024
- Éditeur
- American Association for the Advancement of Science (AAAS)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Copenhagen Structural Biology and NMR Laboratory pays non établi dans la noticeUniversité ou école supérieure
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St. Jude Children's Research Hospital Department of Structural Biology pays non établi dans la noticeÉtablissement de santé
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Illinois Institute of Technology Department of Physics pays non établi dans la noticeUniversité ou école supérieure
Structural Biology and NMR Laboratory — University of Copenhagen, Department of Structural Biology — St. Jude Children's Research Hospital et Department of Physics — Illinois Institute of Technology.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.