Veterinary perspectives on the urbanization of leishmaniosis in Morocco
Rattachement africain : pt, Maroc. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
BACKGROUND: Leishmaniosis caused by Leishmania infantum, L. major and L. tropica is endemic in Morocco. Growing evidence of both human and canine Leishmania infections in urban centres has been reported. Since many forms of the disease are zoonotic, veterinarians play an important role in leishmaniosis control by intervening at the parasite host level. This study aimed to bring together One Health principles to connect canine and feline leishmaniosis epidemiology within urban centres of Morocco (Rabat and Fez) and assess the level of awareness of Moroccan veterinarians about facing this threat. METHODS: A molecular survey was conducted for Leishmania DNA detection in canine (n = 155) and feline (n = 32) whole-blood samples. Three conventional polymerase chain reaction (PCR) protocols were implemented. The first PCR aimed at identifying infected animals by targeting Leishmania spp. kinetoplast minicircle DNA (kDNA). The second and third PCR targeted the Leishmania internal transcribed spacer region (ITS-1) and the Leishmania small subunit ribosomal RNA (SSUrRNA) gene, respectively, aiming at identification of the infecting species after Sanger sequencing-positive amplicons. Total immunoglobulin G (IgG) against Leishmania spp. was evaluated in 125 dogs by enzyme-linked immunosorbent assays (ELISA) using an in-house protocol, including three Leishmania-specific antigens (SPLA, rKDDR and LicTXNPx). Sera from 25 cats were screened for total IgG to Leishmania spp. by an indirect immunofluorescence antibody test (IFAT). An online questionnaire was presented to Moroccan veterinarians addressing their knowledge and practices towards animal leishmaniosis. RESULTS: Overall, 19.4% of the dogs tested positive for Leishmania kDNA and ITS-1 and sequencing revealed infection with L. infantum among PCR-positive dogs. These animals presented a wide range of ELISA seropositivity results (16.7%, 34.9% and 51.6%) according to the tested antigens (rKDDR, SPLA and LicTXNPx, respectively). Use of kDNA-PCR revealed 12.5% cats positive to Leishmania spp. otherwise found to be seronegative by IFAT. CONCLUSIONS: A considerable prevalence of infection was identified in dogs from urban centres of Morocco. Additionally, this is the first report of feline infection with Leishmania spp. in this country and in urban settings. Moroccan veterinarians are aware that animal leishmaniosis is endemic in Morocco, representing a public health threat, and are knowledgeable about canine leishmaniosis diagnosis and treatment.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Veterinary perspectives on the urbanization of leishmaniosis in Morocco
- Date Crossref
- 19/08/2024
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Universidade do Porto pays non établi dans la noticeUniversité ou école supérieure
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Institut Agronomique et Vétérinaire Hassan II Unité de Parasitologie et Maladies Parasitaires Rabat, Maroc (code pays fourni par la source)Université ou école supérieure
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University of Trás-os-Montes and Alto Douro pays non établi dans la noticeUniversité ou école supérieure
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Association for the Development of Douro Viticulture pays non établi dans la noticeOrganisation à but non lucratif
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University of Porto Institute for Research and Innovation in Health pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Pharmacy Microbiology Laboratory pays non établi dans la noticeUniversité ou école supérieure
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Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS) pays non établi dans la noticeStructure de recherche
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University of Trás-os-Montes e Alto Douro Department of Veterinary Sciences pays non établi dans la noticeUniversité ou école supérieure
Universidade do Porto, Unité de Parasitologie et Maladies Parasitaires — Institut Agronomique et Vétérinaire Hassan II (Rabat, Maroc) et University of Trás-os-Montes and Alto Douro, avec 5 autres affiliations. Pays d’affiliation : Maroc.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.