DNA-DISK: Automated end-to-end data storage via enzymatic single-nucleotide DNA synthesis and sequencing on digital microfluidics
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Le résumé fourni par la source
In the age of information explosion, the exponential growth of digital data far exceeds the capacity of current mainstream storage media. DNA is emerging as a promising alternative due to its higher storage density, longer retention time, and lower power consumption. To date, commercially mature DNA synthesis and sequencing technologies allow for writing and reading of information on DNA with customization and convenience at the research level. However, under the disconnected and nonspecialized mode, DNA data storage encounters practical challenges, including susceptibility to errors, long storage latency, resource-intensive requirements, and elevated information security risks. Herein, we introduce a platform named DNA-DISK that seamlessly streamlined DNA synthesis, storage, and sequencing on digital microfluidics coupled with a tabletop device for automated end-to-end information storage. The single-nucleotide enzymatic DNA synthesis with biocapping strategy is utilized, offering an ecofriendly and cost-effective approach for data writing. A DNA encapsulation using thermo-responsive agarose is developed for on-chip solidification, not only eliminating data clutter but also preventing DNA degradation. Pyrosequencing is employed for in situ and accurate data reading. As a proof of concept, DNA-DISK successfully stored and retrieved a musical sheet file (228 bits) with lower write-to-read latency (4.4 min of latency per bit) as well as superior automation compared to other platforms, demonstrating its potential to evolve into a DNA Hard Disk Drive in the future.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- DNA-DISK: Automated end-to-end data storage via enzymatic single-nucleotide DNA synthesis and sequencing on digital microfluidics
- Date Crossref
- 15/08/2024
- Éditeur
- National Academy of Sciences
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Xiamen University pays non établi dans la noticeUniversité ou école supérieure
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Tan Kah Kee Innovation Laboratory pays non établi dans la noticeStructure de recherche
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Chinese Academy of Sciences pays non établi dans la noticeOrganisme public
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Tianjin Institute of Industrial Biotechnology pays non établi dans la noticeOrganisation à but non lucratif
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College of Chemistry and Chemical Engineering Key Laboratory of Spectrochemical Analysis and Instrumentation pays non établi dans la noticeUniversité ou école supérieure
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Zhonghe Gene Technology Co. pays non établi dans la noticeOrganisation à but non lucratif
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Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM) pays non établi dans la noticeStructure de recherche
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Key Laboratory of Engineering Biology for Low-Carbon Manufacturing pays non établi dans la noticeStructure de recherche
Xiamen University, Tan Kah Kee Innovation Laboratory et Chinese Academy of Sciences, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.