Time-resolved scRNA-seq reveals transcription dynamics of polarized macrophages with influenza A virus infection and antigen presentation to T cells
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Le résumé fourni par la source
Throughout history, the influenza A virus has caused numerous devastating global pandemics. Macrophages, as pivotal innate immune cells, exhibit a wide range of immune functions characterized by distinct polarization states, reflecting their intricate heterogeneity. In this study, we employed the time-resolved single-cell sequencing technique coupled with metabolic RNA labelling to elucidate the dynamic transcriptional changes in distinct polarized states of bone marrow-derived macrophages (BMDMs) upon infection with the influenza A virus. Our approach not only captures the temporal dimension of transcriptional activity, which is lacking in conventional scRNA-seq methods, but also reveals that M2-polarized Arg1_macrophages is the sole state supporting successful replication of influenza A virus. Furthermore, we identified distinct antigen presentation capabilities to CD4+ T and CD8+ T cells across diverse polarized states of macrophages. Notably, the M1 phenotype, exhibited by both bone marrow-derived macrophages (BMDMs) and murine alveolar macrophages (AMs), demonstrated superior conventional and cross-presentation abilities for exogenous antigens, with a particular emphasis on cross-presentation capacity. Additionally, as CD8+ T cell differentiation progressed, M1 polarization exhibited an enhanced capacity for cross-presentation. All three phenotypes of BMDMs, including M1, demonstrated robust presentation of CD4+ regulatory T cells, while displaying limited ability to present naive CD4+ T cells. These findings offer novel insights into the immunological regulatory mechanisms governing distinct polarized states of macrophages, particularly their roles in restricting the replication of influenza A virus and modulating antigen-specific T cell responses through innate immunity.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Time-resolved scRNA-seq reveals transcription dynamics of polarized macrophages with influenza A virus infection and antigen presentation to T cells
- Date Crossref
- 01/09/2024
- Éditeur
- Informa UK Limited
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Chinese Academy of Medical Sciences & Peking Union Medical College Chinese Academy of Medical Sciences pays non établi dans la noticeUniversité ou école supérieure
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China-Japan Friendship Hospital pays non établi dans la noticeÉtablissement de santé
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Peking Union Medical College Hospital pays non établi dans la noticeÉtablissement de santé
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Center for Life Sciences pays non établi dans la noticeUniversité ou école supérieure
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Tsinghua University THU-PKU Joint Center for Life Sciences pays non établi dans la noticeUniversité ou école supérieure
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Capital Medical University Department of Infectious Disease pays non établi dans la noticeUniversité ou école supérieure
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Beijing Friendship Hospital pays non établi dans la noticeÉtablissement de santé
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National Institute for Viral Disease Control and Prevention pays non établi dans la noticeOrganisme public
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National Clinical Research Center for Respiratory Diseases Department of Pulmonary and Critical Care Medicine pays non établi dans la noticeStructure de recherche
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Chinese Academy of Medical Sciences and Peking Union Medical College Institute of Respiratory Medicine pays non établi dans la noticeUniversité ou école supérieure
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Graduate School of Peking Union Medical College pays non établi dans la noticeUniversité ou école supérieure
Chinese Academy of Medical Sciences — Chinese Academy of Medical Sciences & Peking Union Medical College, China-Japan Friendship Hospital et Peking Union Medical College Hospital, avec 8 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.