GC–MS analysis, molecular docking, and apoptotic-based cytotoxic effect of Caladium lindenii Madison extracts toward the HeLa cervical cancer cell line
Rattachement africain : pk. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Utilizing medicinal plants and other natural resources to prevent different types of human cancers is the prime focus of attention. Cervical cancer in women ranks as the fourth most common type of malignancy. The current study used gas chromatography-mass spectrometry (GC–MS) to identify the active phytochemical constituents from Caladium lindenii leaf extracts using ethanol (ECL) and n-hexane (HCL) solvents. Plant extracts were tested for potential cytotoxic effects on HeLa and HEK-293 T cells using the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) and the crystal violet assays. SYBR Green-based real-time PCR was performed to assess the mRNA expression profile of the apoptosis biomarkers ( BCL-2 and TP53 ). The molecular interaction of the compounds with the targeted proteins (TP53, BCL2, EGFR, and HER2) was determined using molecular docking. GC–MS analysis revealed a total of 93 compounds in both extracts. The ECL extract significantly reduced the proliferation of HeLa cervical cancer cells, with an IC 50 value of 40 µg/mL, while HEK-293 T cells showed less effect (IC 50 = 226 µg/mL). The quantitative RT-PCR gene expression analysis demonstrated the ethanol extract regulated TP53 and BCL2 mRNA expressions in treated cancer cell samples. Heptanediamide, N,Nʹ-di-benzoyloxy-(− 10.1) is the best-docked ligand with a TP53 target found in the molecular docking study, whereas EGFR / Clionasterol had the second highest binding affinity (− 9.7), followed by EGFR/Cycloeucalenol (− 9.6). It is concluded that ECL extract has promising anti-cervical cancer potential and might be valued for developing new plant-derived anticancer agents after further investigations.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- GC–MS analysis, molecular docking, and apoptotic-based cytotoxic effect of Caladium lindenii Madison extracts toward the HeLa cervical cancer cell line
- Date Crossref
- 08/08/2024
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Pakistan Council of Scientific & Industrial Research pays non établi dans la noticeOrganisme public
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University of Lahore Institute of Molecular Biology and Biotechnology pays non établi dans la noticeUniversité ou école supérieure
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Punjab Medical College pays non établi dans la noticeUniversité ou école supérieure
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University of the Punjab pays non établi dans la noticeUniversité ou école supérieure
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Pakistan Council of Scientific and Industrial Research (PCSIR) pays non établi dans la noticeInstitution
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Faisalabad Medical University Department of Physiology pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Agricultural Sciences Department of Plant Breeding and Genetics pays non établi dans la noticeUniversité ou école supérieure
Pakistan Council of Scientific & Industrial Research, Institute of Molecular Biology and Biotechnology — University of Lahore et Punjab Medical College, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.