Identificacion de polimorfismos en el gen mieloperoxidasa y su papel en candidiasis vulvovaginal recurrente por Candida spp., aisladas de pacientes de Bogota DC

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Introduction: Myeloperoxidase (MPO) is an antimicrobial protein present in the azuro- philic granules of neutrophils, its action falls when it is conjugated in the phagosome with hydrogen peroxide, resulting in toxic substances for phagocytosed microorganisms. Seve- ral authors have described that recurrent vulvovaginal infections by Candida spp., are rela- ted to primary MPO deficiency, which is an autosomal recessive disorder, due to polymor- phisms associated with posttranslational processing or pre-translational errors. Objective: To identify the relationship between the two MPO polymorphisms (Y173C-M251T) with a higher probability of presenting vulvovaginal infection. Materials and methods: 27 blood samples were collected from women between 20-45 years old diagnosed with vul- vovaginal candidiasis in order to analyze the complete blood count, Wright stain, leuko- cyte peroxidase stain and perform DNA extraction to assess the quantity and quality of genetic material. Subsequently, primers for the mpo gene were designed by bioinformatic tools and the conditions to standardize conventional PCR were established. Amplified products were sequenced by capillary electrophoresis (CES) to identify polymorphisms. Results: The polymorphisms of interest were not found in any sample. However, nucleoti- de changes were found in exons 4 and 6 of the fragment studied in two samples analyzed, indicating that this region may present genetic variability. Conclusion: The present study is a first approximation in Colombia on the mpo gene polymorphisms from patients with vulvovaginal Candidiasis in Bogotá D.C. Future studies with a more representative num- ber of samples will give us stronger indications of the genetic variability that the mpo gene may present.

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