Extracellular vesicles promote migration despite BRAF inhibitor treatment in malignant melanoma cells
Rattachement africain : hu. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Extracellular vesicles (EVs) constitute a vital component of intercellular communication, exerting significant influence on metastasis formation and drug resistance mechanisms. Malignant melanoma (MM) is one of the deadliest forms of skin cancers, because of its high metastatic potential and often acquired resistance to oncotherapies. The prevalence of BRAF mutations in MM underscores the importance of BRAF-targeted therapies, such as vemurafenib and dabrafenib, alone or in combination with the MEK inhibitor, trametinib. This study aimed to elucidate the involvement of EVs in MM progression and ascertain whether EV-mediated metastasis promotion persists during single agent BRAF (vemurafenib, dabrafenib), or MEK (trametinib) and combined BRAF/MEK (dabrafenib/trametinib) inhibition.Using five pairs of syngeneic melanoma cell lines, we assessed the impact of EVs - isolated from their respective supernatants - on melanoma cell proliferation and migration. Cell viability and spheroid growth assays were employed to evaluate proliferation, while migration was analyzed through mean squared displacement (MSD) and total traveled distance (TTD) measurements derived from video microscopy and single-cell tracking.Our results indicate that while EV treatments had remarkable promoting effect on cell migration, they exerted only a modest effect on cell proliferation and spheroid growth. Notably, EVs demonstrated the ability to mitigate the inhibitory effects of BRAF inhibitors, albeit they were ineffective against a MEK inhibitor and the combination of BRAF/MEK inhibitors. In summary, our findings contribute to the understanding of the intricate role played by EVs in tumor progression, metastasis, and drug resistance in MM.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Extracellular vesicles promote migration despite BRAF inhibitor treatment in malignant melanoma cells
- Date Crossref
- 22/05/2024
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Pázmány Péter Catholic University pays non établi dans la noticeUniversité ou école supérieure
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Institute of Materials and Environmental Chemistry pays non établi dans la noticeStructure de recherche
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HUN-REN Research Centre for Natural Sciences pays non établi dans la noticeStructure de recherche
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Semmelweis University Department of Genetics pays non établi dans la noticeUniversité ou école supérieure
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Montavid Thermodynamic Research Group pays non établi dans la noticeStructure de recherche
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Faculty of Information Technology and Bionics pays non établi dans la noticeUniversité ou école supérieure
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ELKH-SE Translational Extracellular Vesicle Research Group pays non établi dans la noticeInstitution
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HCEMM-SE Extracellular Vesicle Research Group pays non établi dans la noticeInstitution
Pázmány Péter Catholic University, Institute of Materials and Environmental Chemistry et HUN-REN Research Centre for Natural Sciences, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.