Function investigation of p11.5 in ASFV infection
Rattachement africain : cn, mv. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Virus replication relies on complex interactions between viral proteins. In the case of African swine fever virus (ASFV), only a few such interactions have been identified so far. In this study, we demonstrate that ASFV protein p72 interacts with p11.5 using co-immunoprecipitation and liquid chromatography-mass spectrometry (LC-MS). It was found that protein p72 interacts specifically with p11.5 at sites amino acids (aa) 1-216 of p72 and aa 1-68 of p11.5. To assess the importance of p11.5 in ASFV infection, we developed a recombinant virus (ASFVGZΔA137R) by deleting the A137R gene from the ASFVGZ genome. Compared with ASFVGZ, the infectious progeny virus titers of ASFVGZΔA137R were reduced by approximately 1.0 logs. In addition, we demonstrated that the growth defect was partially attributable to a higher genome copies-to-infectious virus titer ratios produced in ASFVGZΔA137R-infected MA104 cells than in those infected with ASFVGZ. This finding suggests that MA104 cells infected with ASFVGZΔA137R may generate larger quantities of noninfectious particles. Importantly, we found that p11.5 did not affect virus-cell binding or endocytosis. Collectively, we show for the first time the interaction between ASFV p72 and p11.5. Our results effectively provide the relevant information of the p11.5 protein. These results extend our understanding of complex interactions between viral proteins, paving the way for further studies of the potential mechanisms and pathogenesis of ASFV infection.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Function investigation of p11.5 in ASFV infection
- Date Crossref
- 01/06/2024
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Jiangsu Provincial Center for Disease Control and Prevention pays non établi dans la noticeOrganisme public
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Ministry of Education pays non établi dans la noticeOrganisme public
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Yangzhou University Ministry of Education Key Laboratory for Avian Preventive Medicine pays non établi dans la noticeUniversité ou école supérieure
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Shanghai Veterinary Research Institute pays non établi dans la noticeStructure de recherche
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South China Agricultural University pays non établi dans la noticeUniversité ou école supérieure
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Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses pays non établi dans la noticeInstitution
Jiangsu Provincial Center for Disease Control and Prevention, Ministry of Education et Ministry of Education Key Laboratory for Avian Preventive Medicine — Yangzhou University, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.