A Novel N-heterocyclic Carbene Compound Exhibits Anticancer Properties by Inducing Apoptosis, Inhibiting Cell Migration, and Improving Standard Therapeutic Efficacy in Human Colorectal Cancer Cell Lines
Rattachement africain : sa, Tunisie, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Colorectal cancer (CRC) remains a major global health issue. Although chemotherapy is the first-line treatment, its effectiveness is limited due to drug resistance developed in CRC. To overcome resistance and improve the prognosis of CRC patients, investigation for new therapeutic approaches is necessary. Using human colorectal adenocarcinoma (HT29) and metastatic CRC (SW620) cell lines, the potential anticancer properties of a newly synthesized compound 1-(Isobutyl)-3-(4-methylbenzyl) benzimidazolium chloride (IMBZC) were evaluated by performing MTT cytotoxicity, cell migration, and colony formation assays, as well as by monitoring apoptosis-related protein and gene expression using Western blot and reverse transcription-quantitative polymerase chain reaction technologies. Tested at various concentrations, the half-maximal inhibitory concentrations (IC50) of IMBZC on HT29 and SW620 cell growth were determined to be 6.97 μg/mL and 4.89 μg/mL, respectively. IMBZC did not alter cell growth of normal cells HEK293. In addition, IMBZC inhibited cell migration and significantly decreased colony formation, suggesting its promising role in suppressing cancer metastasis. Mechanistic analyzes revealed that IMBZC treatment increased the expression of pro-apoptotic proteins p53 and Bax, while decreasing the expression of anti-apoptotic proteins Bcl-2 and Bcl-xL, thus indicating the induction of apoptosis in IMBZC-treated CRC cells, compared to untreated cells. Additionally, the addition of IMBZC to conventional chemotherapeutic drugs (i.e., 5-fluorouracil, irinotecan, and oxaliplatin) resulted in an increase in the cytotoxic potential of the drugs. In conclusion, the study suggests that IMBZC has substantial anticancer effects against CRC cells through its ability to induce apoptosis, inhibit cancer cell migration and colony formation, and enhance the cytotoxic effects of conventional chemotherapeutic drugs. These findings indicate that IMBZC could be a promising chemotherapeutic drug for the treatment of CRC. Further research should be conducted using in vivo models to confirm the anti-CRC activities of IMBZC.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- A Novel N-heterocyclic Carbene Compound Exhibits Anticancer Properties by Inducing Apoptosis, Inhibiting Cell Migration, and Improving Standard Therapeutic Efficacy in Human Colorectal Cancer Cell Lines
- Date Crossref
- 03/05/2024
- Éditeur
- MDPI AG
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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King Saud University Department of Surgery pays non établi dans la noticeUniversité ou école supérieure
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University of Carthage Research Laboratory of Environmental Sciences and Technologies (LR16ES09) Tunisie (code pays fourni par la source)Université ou école supérieure
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Research and Technology Center of Energy Tunisie (code pays fourni par la source)Structure de recherche
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George Mason University pays non établi dans la noticeUniversité ou école supérieure
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King Saud bin Abdulaziz University for Health Sciences King Abdullah International Medical Research Center (KAIMRC) pays non établi dans la noticeUniversité ou école supérieure
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King Abdullah International Medical Research Center pays non établi dans la noticeStructure de recherche
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National Guard Health Affairs pays non établi dans la noticeÉtablissement de santé
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College of Science Zoology Department pays non établi dans la noticeUniversité ou école supérieure
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College of Medicine Department of Surgery pays non établi dans la noticeUniversité ou école supérieure
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Faculty of the School of Systems Biology Biosciences Department pays non établi dans la noticeUniversité ou école supérieure
Department of Surgery — King Saud University, Research Laboratory of Environmental Sciences and Technologies (LR16ES09) — University of Carthage (Tunisie) et Research and Technology Center of Energy (Tunisie), avec 7 autres affiliations. Pays d’affiliation : Tunisie.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.