Quantitation of Enterovirus A71 Empty and Full Particles by Sedimentation Velocity Analytical Ultracentrifugation
Rattachement africain : cn. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
The enterovirus A71 (EV71) inactivated vaccine is an effective intervention to control the spread of the virus and prevent EV71-associated hand, foot, and mouth disease (HFMD). It is widely administered to infants and children in China. The empty particles (EPs) and full particles (FPs) generated during production have different antigenic and immunogenic properties. However, the antigen detection methods currently used were established without considering the differences in antigenicity between EPs and FPs. There is also a lack of other effective analytical methods for detecting the different particle forms, which hinders the consistency between batches of products. In this study, we analyzed the application of sedimentation velocity analytical ultracentrifugation (SV-AUC) in characterizing the EPs and FPs of EV71. Our results showed that the proportions of the two forms could be quantified simultaneously by SV-AUC. We also determined the repeatability and accuracy of this method and found that both parameters were satisfactory. We assessed SV-AUC for bulk vaccine quality control, and our findings indicated that SV-AUC can be used effectively to analyze the percentage of EPs and FPs and monitor the consistency of the process to ensure the quality of the vaccine.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Quantitation of Enterovirus A71 Empty and Full Particles by Sedimentation Velocity Analytical Ultracentrifugation
- Date Crossref
- 08/04/2024
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Huazhong University of Science and Technology pays non établi dans la noticeUniversité ou école supérieure
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Hubei Provincial Vaccines Technology Innozation Center pays non établi dans la noticeInstitution
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National Engineering Technology Research Center of Combined Vaccines pays non établi dans la noticeStructure de recherche
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National Key Laboratory for Novel Vaccines Research and Development of Emerging Infectious Diseases pays non établi dans la noticeStructure de recherche
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Wuhan Institute of Biological Products Co. pays non établi dans la noticeStructure de recherche
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College of Life Science and Technology The Research Core Facilities for Life Science (HUST) pays non établi dans la noticeUniversité ou école supérieure
Huazhong University of Science and Technology, Hubei Provincial Vaccines Technology Innozation Center et National Engineering Technology Research Center of Combined Vaccines, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.