Fabrication of High-Negatively Charged Bicelle-Mediated Supported Lipid Bilayer
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Le résumé fourni par la source
Supported lipid bilayers (SLBs), two-dimensional lipid films formed on a solid-supporting substrate, serve as models for biomembranes and exhibit remarkable potential in chemistry, biology, and medicine. However, preparing SLBs with highly negatively charged contents on the negatively charged surface by overcoming electrostatic repulsion remains a challenge. Here, a creative bicelle-mediated and divalent cation-free SLB preparation method with the assistance of phosphate-buffered saline (PBS) solution was proposed, which can form the SLBs containing 50% DOPS or 30% CL on the silica surface monitored by a quartz crystal microbalance with dissipation (QCM-D). Results of molecular dynamics (MD) simulation indicate that electrostatic repulsion can be overcome by the increased number of hydrogen bonds caused by the adsorption of dihydrogen phosphate ions onto the headgroups of lipids. In addition, the negatively charged SLB formation was identified to be a three-step kinetic process, which differs from a two-step mechanism in the case of amphoteric SLB. The extra kinetic step can be attributed to the reduction in the number of intermolecular hydrogen bonds and the ordering of water molecules in the hydration layer. This investigation resolves the challenge of fabricating SLB over negatively charged surfaces and offers a fresh perspective on the SLB assembly methodology.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Fabrication of High-Negatively Charged Bicelle-Mediated Supported Lipid Bilayer
- Date Crossref
- 04/04/2024
- Éditeur
- American Chemical Society (ACS)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Jilin University pays non établi dans la noticeUniversité ou école supérieure
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State Key Laboratory of Supramolecular Structure and Materials pays non établi dans la noticeStructure de recherche
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College of Chemistry State Key Laboratory of Supramolecular Structure and Materials pays non établi dans la noticeUniversité ou école supérieure
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School of Life Sciences pays non établi dans la noticeUniversité ou école supérieure
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Key Laboratory of Material Simulation Methods and Software of Ministry of Education pays non établi dans la noticeStructure de recherche
Jilin University, State Key Laboratory of Supramolecular Structure and Materials et State Key Laboratory of Supramolecular Structure and Materials — College of Chemistry, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.