Detection of insulin oligomeric forms by a novel surface plasmon resonance‐diffusion coefficient based approach
Rattachement africain : it. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Insulin is commonly used to treat diabetes and undergoes aggregation at the site of repeated injections in diabetic patients. Moreover, aggregation is also observed during its industrial production and transport and should be avoided to preserve its bioavailability to correctly adjust glucose levels in diabetic patients. However, monitoring the effect of various parameters (pH, protein concentration, metal ions, etc.) on the insulin aggregation and oligomerization state is very challenging. In this work, we have applied a novel Surface Plasmon Resonance (SPR)-based experimental approach to insulin solutions at various experimental conditions, monitoring how its diffusion coefficient is affected by pH and the presence of metal ions (copper and zinc) with unprecedented sensitivity, precision, and reproducibility. The reported SPR method, hereby applied to a protein for the first time, besides giving insight into the insulin oligomerization and aggregation phenomena, proved to be very robust for determining the diffusion coefficient of any biomolecule. A theoretical background is given together with the software description, specially designed to fit the experimental data. This new way of applying SPR represents an innovation in the bio-sensing field and expanding the potentiality of commonly used SPR instruments well over the canonical investigation of biomolecular interactions.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Detection of insulin oligomeric forms by a novel surface plasmon resonance‐diffusion coefficient based approach
- Date Crossref
- 19/03/2024
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Fondazione G.B. Bietti pays non établi dans la noticeOrganisation à but non lucratif
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Istituti di Ricovero e Cura a Carattere Scientifico pays non établi dans la noticeÉtablissement de santé
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University of Catania Dipartimento di Scienze Chimiche pays non établi dans la noticeUniversité ou école supérieure
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IRCCS‐Fondazione Bietti Rome Italy pays non établi dans la noticeInstitution
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IRCCS-Fondazione Bietti pays non établi dans la noticeInstitution
Fondazione G.B. Bietti, Istituti di Ricovero e Cura a Carattere Scientifico et Dipartimento di Scienze Chimiche — University of Catania, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.