CD4 + CD11b + T cells infiltrate and aggravate the traumatic brain injury depending on brain‐to‐cervical lymph node signaling
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Le résumé fourni par la source
Abstract Aim We aim to identify the specific CD4+ T‐cell subtype influenced by brain‐to‐CLN signaling and explore their role during the acute phase of traumatic brain injury (TBI). Method Cervical lymphadenectomy or cervical afferent lymphatic ligation was performed before TBI. Cytokine array and western blot were used to detect cytokines, while the motor function was assessed using mNss and rotarod test. CD4+ T‐cell subtypes in blood, brain, and CLNs were analyzed with Cytometry by time‐of‐flight analysis (CyTOF) or fluorescence‐activated cell sorting (FACS). Brain edema and volume changes were measured by 9.4T MRI. Neuronal apoptosis was evaluated by terminal deoxynucleotidyl transferase‐mediated dUTP nick end labeling (TUNEL) staining. Results Cervical lymphadenectomy and ligation of cervical lymphatic vessels resulted in a decreased infiltration of CD4+ T cells, specifically CD11b‐positive CD4+ T cells, within the affected region. The population of CD4+CD11b+ T cells increased in ligated CLNs, accompanied by a decrease in the average fluorescence intensity of sphingosine‐1‐phosphate receptor‐1 (S1PR1) on these cells. Administration of CD4+CD11b+ T cells sorted from CLNs into the lateral ventricle reversed the attenuated neurologic deficits, brain edema, and lesion volume following cervical lymphadenectomy. Conclusion The infiltration of CD4+CD11b+ T cells exacerbates secondary brain damage in TBI, and this process is modulated by brain‐to‐CLN signaling.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- <scp>CD4</scp><sup>+</sup><scp>CD11b</scp><sup>+</sup> T cells infiltrate and aggravate the traumatic brain injury depending on brain‐to‐cervical lymph node signaling
- Date Crossref
- 01/03/2024
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Tianjin Medical University General Hospital Tianjin Neurological Institute pays non établi dans la noticeÉtablissement de santé
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State Key Laboratory of Chemical Engineering pays non établi dans la noticeStructure de recherche
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Tianjin Medical University Department of Neurosurgery pays non établi dans la noticeUniversité ou école supérieure
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Tianjin First Center Hospital pays non établi dans la noticeÉtablissement de santé
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State Key Laboratory of Experimental Hematology Tianjin China pays non établi dans la noticeStructure de recherche
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Department of Neurosurgery Tianjin First Central Hospital Tianjin China Department of Neurosurgery pays non établi dans la noticeÉtablissement de santé
Tianjin Neurological Institute — Tianjin Medical University General Hospital, State Key Laboratory of Chemical Engineering et Department of Neurosurgery — Tianjin Medical University, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.