Identification and functional activity of Nik related kinase (NRK) in benign hyperplastic prostate
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Le résumé fourni par la source
Abstract Objective Benign prostatic hyperplasia (BPH) is common in elder men. The current study aims to identify differentially expressed genes (DEGs) in hyperplastic prostate and to explore the role of Nik related kinase (NRK) in BPH. Methods Four datasets including three bulk and one single cell RNA-seq (scRNA-seq) were obtained to perform integrated bioinformatics. Cell clusters and specific metabolism pathways were analyzed. The localization, expression and functional activity of NRK was investigated via RT-PCR, western-blot, immunohistochemical staining, flow cytometry, wound healing assay, transwell assay and CCK-8 assay. Results A total of 17 DEGs were identified by merging three bulk RNA-seq datasets. The findings of integrated single-cell analysis showed that NRK remarkably upregulated in fibroblasts and SM cells of hyperplasia prostate. Meanwhile, NRK was upregulated in BPH samples and localized almost in stroma. The expression level of NRK was significantly correlated with IPSS and Qmax of BPH patients. Silencing of NRK inhibited stromal cell proliferation, migration, fibrosis and EMT process, promoted apoptosis and induced cell cycle arrest, while overexpression of NRK in prostate epithelial cells showed opposite results. Meanwhile, induced fibrosis and EMT process were rescued by knockdown of NRK. Furthermore, expression level of NRK was positively correlated with that of α-SMA, collagen-I and N-cadherin, negatively correlated with that of E-cadherin. Conclusion Our novel data identified NRK was upregulated in hyperplastic prostate and associated with prostatic stromal cell proliferation, apoptosis, cell cycle, migration, fibrosis and EMT process. NRK may play important roles in the development of BPH and may be a promising therapeutic target for BPH/LUTS.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Identification and functional activity of Nik related kinase (NRK) in benign hyperplastic prostate
- Date Crossref
- 09/03/2024
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Xijing Hospital Department of Urology pays non établi dans la noticeÉtablissement de santé
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Air Force Medical University Department of Physiology and Pathophysiology pays non établi dans la noticeUniversité ou école supérieure
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Wuhan University Department of Urology pays non établi dans la noticeUniversité ou école supérieure
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Zhongnan Hospital of Wuhan University pays non établi dans la noticeÉtablissement de santé
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Air Force Engineering University pays non établi dans la noticeUniversité ou école supérieure
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Ninth Hospital of Xi'an Department of Orthopedics pays non établi dans la noticeÉtablissement de santé
Department of Urology — Xijing Hospital, Department of Physiology and Pathophysiology — Air Force Medical University et Department of Urology — Wuhan University, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.