Colonic epithelial hypoxia remains constant during the progression of diabetes in male UC Davis type 2 diabetes mellitus rats
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Le résumé fourni par la source
INTRODUCTION: Colonocyte oxidation of bacterial-derived butyrate has been reported to maintain synergistic obligate anaerobe populations by reducing colonocyte oxygen levels; however, it is not known whether this process is disrupted during the progression of type 2 diabetes. Our aim was to determine whether diabetes influences colonocyte oxygen levels in the University of California Davis type 2 diabetes mellitus (UCD-T2DM) rat model. RESEARCH DESIGN AND METHODS: Age-matched male UCD-T2DM rats (174±4 days) prior to the onset of diabetes (PD, n=15), within 1 month post-onset (RD, n=12), and 3 months post-onset (D3M, n=12) were included in this study. Rats were administered an intraperitoneal injection of pimonidazole (60 mg/kg body weight) 1 hour prior to euthanasia and tissue collection to estimate colonic oxygen levels. Colon tissue was fixed in 10% formalin, embedded in paraffin, and processed for immunohistochemical detection of pimonidazole. The colonic microbiome was assessed by 16S gene rRNA amplicon sequencing and content of short-chain fatty acids was measured by liquid chromatography-mass spectrometry. RESULTS: (false discovery rate <0.05) genera in colon contents. The intensity of pimonidazole staining of colonic epithelia did not differ across groups (p=0.37). Colon content concentrations of acetate and propionate also did not differ across UCD-T2DM groups; however, colonic butyric acid levels were higher in D3M rats relative to PD rats (p<0.01). CONCLUSIONS: The advancement of diabetes in UCD-T2DM rats was associated with an increase in facultative anaerobes; however, this was not explained by changes in colonocyte oxygen levels. The mechanisms underlying shifts in gut microbe populations associated with the progression of diabetes in the UCD-T2DM rat model remain to be identified.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Colonic epithelial hypoxia remains constant during the progression of diabetes in male UC Davis type 2 diabetes mellitus rats
- Date Crossref
- 01/03/2024
- Éditeur
- BMJ
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Arkansas Children's Nutrition Center pays non établi dans la noticeOrganisme public
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University of Arkansas for Medical Sciences Department of Pediatrics pays non établi dans la noticeUniversité ou école supérieure
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University of California Department of Nutrition pays non établi dans la noticeUniversité ou école supérieure
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Arkansas Children's Hospital pays non établi dans la noticeÉtablissement de santé
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Arkansas Children's Research Institute Center for Translational Pediatric Research pays non établi dans la noticeOrganisation à but non lucratif
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Lincoln Memorial University pays non établi dans la noticeUniversité ou école supérieure
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Virginia Tech pays non établi dans la noticeUniversité ou école supérieure
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School of Veterinary Medicine Department of Molecular Biosciences pays non établi dans la noticeUniversité ou école supérieure
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College of Osteopathic Medicine pays non établi dans la noticeUniversité ou école supérieure
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Virginia Polytechnic Institute and State University Department of Biomedical Science and Pathobiology pays non établi dans la noticeUniversité ou école supérieure
Arkansas Children's Nutrition Center, Department of Pediatrics — University of Arkansas for Medical Sciences et Department of Nutrition — University of California, avec 7 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.