Preparation and Crystallographic Analysis of a Complex of SARS-CoV-2 S-Protein Receptor-Binding Domain with a Virus-Neutralizing Nanoantibody
Rattachement africain : ru. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Abstract Coronavirus SARS-CoV-2 was the cause of global pandemic COVID-19 that has claimed millions of lives around the world. One of the approaches to the therapy of severe forms of COVID-19 is based on the use of virus-neutralizing antibodies against the receptor-binding domain (RBD) of the virus spike glycoprotein (S). Detailed structural data on particular epitopes recognized by these virus-neutralizing antibodies is an important aspect of the improvement of available antibodies in order to extend the range of recognized virus strains. A stoichiometric complex of the virus-neutralizing single-domain antibody P2C5 and the glycosylated form of SARS-CoV-2 S protein RBD was obtained. Despite numerous conditions in which the crystal growth of the complex was observed, these crystals showed poor diffraction, apparently because of heterogeneity associated with the glycosylation of RBD. With the aim of improving the crystal quality, deglycosylase PNGase F was produced in a bacterial expression system and deglycosylation of RBD was performed. Despite low solubility of the deglycosylated form of RBD, it retains the ability to bind to the nanoantibody P2C5, resulting in the formation of a stable heterodimeric complex during gel filtration. The crystals of this complex were obtained. They showed diffraction to a similar resolution of ~4 Å on a laboratory X-ray diffractometer and at a synchrotron radiation source. The symmetry space group, the unit cell parameters of the crystal, and its protein composition were determined.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Preparation and Crystallographic Analysis of a Complex of SARS-CoV-2 S-Protein Receptor-Binding Domain with a Virus-Neutralizing Nanoantibody
- Date Crossref
- 01/12/2023
- Éditeur
- Pleiades Publishing Ltd
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Russian Academy of Sciences pays non établi dans la noticeOrganisme public
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Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of Sciences pays non établi dans la noticeStructure de recherche
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N.D. Zelinsky Institute of Organic Chemistry pays non établi dans la noticeStructure de recherche
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Ministry of Health of the Russian Federation pays non établi dans la noticeOrganisme public
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Bach Institute of Biochemistry pays non établi dans la noticeStructure de recherche
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Gamaleya National Research Center for Epidemiology and Microbiology pays non établi dans la noticeStructure de recherche
Russian Academy of Sciences, Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of Sciences et N.D. Zelinsky Institute of Organic Chemistry, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.