Abstract TMP120: Contralesional Corticospinal Tract Reinnervates Cervical Spinal Anatomical Regions With Highest Pre-Lesion Synapse Density After Unilateral M1 Stroke in Mice
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Le résumé fourni par la source
Introduction: Stroke leaves many patients with significant motor impairment. Following ischemic lesions of the rodent primary motor cortex, sprouting of the contralesional corticospinal tract (cCST) into the injured hemicord is associated with improved motor recovery. In adult mice, most corticospinal neurons project to spinal interneurons, and few synapse directly on alpha motor neurons. We examined the distribution of newly formed corticospinal synapses in the mouse spinal cord, using genetically encoded tracers, 3D microscopy, and a spinal atlas with machine learning-based automated classification and registration. Hypothesis: We tested the hypothesis that cCST neurons innervate homotopic regions in the injured hemicord after unilateral motor cortex stroke in adult mice. Method: We induced a photothrombotic motor cortex stroke or performed sham surgery (n=4 per group) in 8-11 week old male C57/B6 mice and administered a contralesional motor cortex injection of an anterograde adeno-associated virus expressing both membrane-targeted tdTomato and synaptically-targeted eGFP. Cervical spinal cords were subjected to volumetric imaging via serial two-photon tomography (TissueCyte 1000) to characterize synaptic and axonal density of cCST collaterals in whole cervical spinal cords in both stroke and sham mice. Unbiased, global quantification of axonal and synaptic density across the entire cervical cord was accomplished by the development of a custom automated image analysis pipeline, incorporating a novel 3D spinal cord reference volume, published spinal cord annotations comprising 47 distinct anatomical regions (SpinalJ), and machine learning based pixel classification. Results: We observed cCST synapses in the 6-week post-stroke injured hemicord innervating lamina IV & IX most densely at C7 & C8 (See Figure). Conclusions: Contralesional CST reinnervation of the denervated hemicord is targeted to both lamina and cervical level specific.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Abstract TMP120: Contralesional Corticospinal Tract Reinnervates Cervical Spinal Anatomical Regions With Highest Pre-Lesion Synapse Density After Unilateral M1 Stroke in Mice
- Date Crossref
- 01/02/2024
- Éditeur
- Ovid Technologies (Wolters Kluwer Health)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
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The University of Texas at San Antonio Health Science Center pays non établi dans la noticeUniversité ou école supérieure
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The University of Texas Health Science Center at Houston pays non établi dans la noticeUniversité ou école supérieure
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Southwestern University pays non établi dans la noticeUniversité ou école supérieure
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The University of Texas Southwestern Medical Center pays non établi dans la noticeÉtablissement de santé
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Southwestern Medical Center pays non établi dans la noticeÉtablissement de santé
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UT Health Science Cntr San Antonio pays non établi dans la noticeInstitution
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Univ of Texas Southwestern Med Cntr pays non établi dans la noticeUniversité ou école supérieure
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UT Southwestern Med Cntr Ariana Nawaby UT Southwestern Med Cntr, Tunisie (ville ou établissement reconnu, pays non nommé)Institution
The University of Texas at San Antonio Health Science Center, The University of Texas Health Science Center at Houston et Southwestern University, avec 5 autres affiliations. Pays d’affiliation : Tunisie.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.