Higher concentration of P7C3 than required for neuroprotection suppresses renal cell carcinoma growth and metastasis
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Le résumé fourni par la source
Background: P7C3 is a novel compound that has been widely applied in neurodegenerative diseases and nerve injury repair.Here, we show that higher concentrations of P7C3 than are required for in vivo neuroprotection have the novel function of suppressing renal cell carcinoma (RCC) proliferation and metastasis.Methods: Colony formation, CCK-8 and EdU assay were applied to evaluate RCC cell proliferation.Wound healing and transwell assay were used to measure RCC cell migration and invasion.Flow cytometry assay was employed to detect RCC cell apoptosis and cell cycle.qRT-PCR assay was carried out to measure ribonucleotide reductase subunit M2 (RRM2) mRNA expression level, while western blot assay was utilized to detect the expression level of target proteins.RCC cell growth in vivo was determined by xenografts in mice.Results: We observed that high concentrations of P7C3 could restrain the proliferation and metastasis of RCC cells and promote cell apoptosis.Mechanistically, this new effect of higher dose of P7C3 was associated with reduced expression of RRM2, and the beneficial efficacy of P7C3 in RCC was blocked when suppression of RRM2 was prevented.When RRM2 suppression was permitted, the cGAS-STING pathway was activated by virtue of RRM2/Bcl-2/Bax signaling.Lastly, intraperitoneal injection of this high level of P7C3 in mice potently inhibited tumor growth.Conclusion: In conclusion, we show here that P7C3 that exerts an anti-cancer effect in RCC.Our study indicated that P7C3 might act as a novel drug for RCC in the future.The regulatory signal pathway RRM2/Bcl-2/BAX/cGAS-STING might present novel insight to the potential mechanism of RCC development.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Higher concentration of P7C3 than required for neuroprotection suppresses renal cell carcinoma growth and metastasis
- Date Crossref
- 01/01/2024
- Éditeur
- Ivyspring International Publisher
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Peking University Shenzhen Hospital Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics pays non établi dans la noticeÉtablissement de santé
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University of Hong Kong - Shenzhen Hospital pays non établi dans la noticeÉtablissement de santé
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Peking University Department of Urology pays non établi dans la noticeUniversité ou école supérieure
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Peking University First Hospital pays non établi dans la noticeÉtablissement de santé
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Shanghai Jiao Tong University Department of Urology pays non établi dans la noticeUniversité ou école supérieure
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Capital Medical University Department of Urology pays non établi dans la noticeUniversité ou école supérieure
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Shanghai First People's Hospital pays non établi dans la noticeÉtablissement de santé
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Beijing Friendship Hospital pays non établi dans la noticeÉtablissement de santé
Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics — Peking University Shenzhen Hospital, University of Hong Kong - Shenzhen Hospital et Department of Urology — Peking University, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.