The expression changes of PD-L1 and immune response mediators are related to the severity of primary bone tumors
Rattachement africain : ir. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
The expression pattern, diagnostic value, and association of PD-L1, IFN-γ and TGF-β with bone tumor type, severity, and relapse are determined in this study. 300 human samples from patients with osteosarcoma, Ewing sarcoma, and GCT were enrolled. The PD-L1 gene and protein expression were assessed by qRT-PCR and immunohistochemistry, respectively. ELISA and flow cytometry was used to detect cytokines and CD4/CD8 T cell percentages, respectively. A considerable increase in PD-L1 level was detected in bone tumor tissues at both gene and protein levels that was considerable in osteosarcoma and Ewing sarcoma. A positive correlation was detected regarding the PD-L1 and tumor metastasis and recurrence in osteosarcoma and Ewing sarcoma. The increased IFN-γ level was detected in patients with metastatic, and recurrent osteosarcoma tumors that were in accordance with the level of TGF-β in these samples. The simultaneous elevation of IFN-γ and TGF-β was detected in Ewing sarcoma and GCT, also the CD4 + /CD8 + ratio was decreased significantly in patients with osteosarcoma compared to GCT tumors. The elevated levels of PD-L1, TGF- β, and IFN-γ were associated with bone tumor severity that can provide insights into the possible role of this axis in promoting immune system escape, suppression, and tumor invasion.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- The expression changes of PD-L1 and immune response mediators are related to the severity of primary bone tumors
- Date Crossref
- 22/11/2023
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Iran University of Medical Sciences Bone and Joint Reconstruction Research Center pays non établi dans la noticeUniversité ou école supérieure
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Tehran University of Medical Sciences Department of Obstetrics and Gynecology pays non établi dans la noticeUniversité ou école supérieure
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School of Medicine Department of Biochemistry pays non établi dans la noticeUniversité ou école supérieure
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School of Public Health Department of Virology pays non établi dans la noticeUniversité ou école supérieure
Bone and Joint Reconstruction Research Center — Iran University of Medical Sciences, Department of Obstetrics and Gynecology — Tehran University of Medical Sciences et Department of Biochemistry — School of Medicine, avec 1 autre affiliation.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.