Epithelial to mesenchymal transition (EMT) changes in patients with non-small cell lung cancer (NSCLC)
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Le résumé fourni par la source
Introduction: Our previous studies have shown active EMT in smokers and COPD patients, which is central to lung cancer development in these patients. Aim: We aim to evaluate EMT changes in extensive patient groups who were diagnosed with NSCLC (adenocarcinoma and squamous cell carcinoma) compared to normal controls (NC). Method: Resected lung tissue from NSCLC patients (n=35), sub-grouped as COPD current and ex-smokers, patients with small airway (SA) disease and normal lung function smokers compared to NC (n=11), were immuno-stained for EMT biomarkers: E-cadherin, N-cadherin, S100A4, Vimentin, and epidermal growth factor receptor (EGFR). Biomarkers were analysed in the SA epithelium and sub-epithelial layers. Tissue analysis was done with microscope-assisted Image-ProPlus 7.0 software. Results: Compared to NC, in all pathological groups, SA wall thickness was significantly increased (p<0.05); SA epithelial E-cadherin expression markedly decreased (p<0.01), whereas N-cadherin, Vimentin, S100A4, and EGFR expression were notably increased (p<0.01). Vimentin expression in sub-epithelium showed a similar trend to epithelium across all pathological groups (p<0.05). However, such changes were only seen in Rbm for S100A4 (p<0.05). EGFR and N-cadherin expressions in both cancer phenotypes were markedly higher than Vimentin and S100A4 (p<0.0001). EMT markers expression positively correlated to smoking history. Conclusion: EMT is a crucial and active process in NSCLC patients with COPD, resulting in SA remodelling and cancer development. This is the first study to show such changes in broadly phenotyped individuals, suggesting EMT as a key mechanism and novel therapeutic target.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Epithelial to mesenchymal transition (EMT) changes in patients with non-small cell lung cancer (NSCLC)
- Date Crossref
- 09/09/2023
- Éditeur
- European Respiratory Society
- Type
- proceedings-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Tasmania pays non établi dans la noticeUniversité ou école supérieure
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Launceston General Hospital Department of Respiratory Medicine pays non établi dans la noticeÉtablissement de santé
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Royal Hobart Hospital Department of Cardiothoracic Surgery pays non établi dans la noticeÉtablissement de santé
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University of British Columbia Department of Anesthesiology pays non établi dans la noticeUniversité ou école supérieure
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St. Paul's Hospital pays non établi dans la noticeÉtablissement de santé
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School of Health Sciences Department of Laboratory Medicine pays non établi dans la noticeUniversité ou école supérieure
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Launceston Respiratory and Sleep Centre pays non établi dans la noticeInstitution
University of Tasmania, Department of Respiratory Medicine — Launceston General Hospital et Department of Cardiothoracic Surgery — Royal Hobart Hospital, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.