Discussion on the mechanism of action of Salvia miltiorrhiza in the treatment of prostate cancer based on network pharmacology

Le résumé fourni par la source

The purpose of this study was to explore the mechanism of action of Salvia miltiorrhiza in the treatment of prostate cancer (PCa) based on the network pharmacology approach, and to provide a basis for rational clinical use and cellular experiments. The method used in this study was to obtain the main active components and corresponding targets of Salvia miltiorrhiza through the Chinese Medicine System Pharmacology Database and Analysis Platform (TCMSP), Perl, UniProt and other databases. Disease targets for PCa were obtained from GeneCards, OMIM, TTD and DrugBank databases. Using bioinformatics online database mapping tool, Venn diagram was drawn to screen the cross targets of Salvia miltiorrhiza and PCa active components. active components of Salvia miltiorrhiza-PCa related target network and cross target protein interaction (PPI) network were constructed using STRING platform, and Cytoscape 3.8.2 software was used to screen key targets, and R 4.3.0 software was used to perform gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. A total of 58 active components of Salvia miltiorrhiza and their corresponding target genes and 2142 target genes related to PCa were obtained. The results of Venn diagram showed that there were 74 cross targets between the active components of Salvia miltiorrhiza and PCa. Luteolin, tanshinone, quercetin and dihydrosalvia miltiorrhiza lactone may be the important nodes of the active components of Salvia miltiorrhiza-PCa related target network, while MAPK14, MYC, TP53, ESR1 and JUN may be the key targets of PPI network. The results of GO analysis showed that the cellular components of 74 targets of Salvia miltiorrhiza in the treatment of PCa were mainly nuclear, chromatin and cytoplasm, and the biological processes were mainly negative regulation of apoptosis, the response to xenobiotic stimulus, and the positive regulation of cell proliferation, and so on. Molecular functions mainly include enzyme binding process, protein binding process, regulation of protein homodimer activity, binding protein kinase and so on. KEGG pathway enrichment analysis showed that the cross targets were mainly concentrated in cancer signaling pathway, prostate cancer signaling pathway, PI3K-Akt signaling pathway, IL-17 signaling pathway, HIF-1 signaling pathway and so on. This study preliminarily reveals the molecular and cellular pathway mechanism of Salvia miltiorrhiza in the treatment of PCa, which provides a scientific basis for clinical use and a reference direction for follow-up cell experiments.

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