Particulate matter promotes the epithelial to mesenchymal transition in human lung epithelial cells via the ROS pathway.
Rattachement africain : us, cn. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
OBJECTS: Epidemiologic studies have linked exposure to airborne pollutant particulate matter (PM) with increased rates of chronic cardiopulmonary diseases, including asthma and idiopathic pulmonary fibrosis (IPF). Several investigations have suggested that the epithelial-to-mesenchymal transition (EMT) may contribute to the complex pathobiology of environmental exposure-mediated pulmonary fibrosis. The present study was designed to characterize the mechanisms of PM-mediated EMT in human lung epithelial cells (HBECs). METHODS AND RESULTS: PM induced significant dose (0-100 μg/ml) and time (0-72 h)-dependent increases in transforming growth factor β (TGFβ) and fibronectin (FN) protein levels in HBECs lysates. PM-activated TGFβ and FN protein production in HBECs was prevented by the antioxidant N-acetyl-cysteine (NAC, 5 mM). Furthermore, the NF-κB inhibitor BAY11-7082 (5 μM) abolished PM-induced FN production in HBECs. Biomarkers of EMT (ACTA2, SNAIL1 and SNAIL2) in PM-treated HBECs were significantly increased at the mRNA level compared to control cells. CONCLUSIONS: These results demonstrate that PM increases protein levels of TGFβ and FN via reactive oxygen species (ROS)-dependent pathways. In addition, PM exposure induces EMT in human lung epithelial cells, supporting a novel mechanism for PM-induced pulmonary fibrosis.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
Où se fait cette recherche
-
University of Arizona Department of Medicine pays non établi dans la noticeUniversité ou école supérieure
-
Capital Medical University Beijing Chao-Yang Hospital pays non établi dans la noticeÉtablissement de santé
-
Bengbu Medical College Anhui Provincial Key Laboratory of Immunology in Chronic Diseases pays non établi dans la noticeUniversité ou école supérieure
-
Florida International University Center of Translational Science pays non établi dans la noticeUniversité ou école supérieure
Department of Medicine — University of Arizona, Beijing Chao-Yang Hospital — Capital Medical University et Anhui Provincial Key Laboratory of Immunology in Chronic Diseases — Bengbu Medical College, avec 1 autre affiliation.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.