Editorial: Methods and applications in vascular physiology: 2022

Le résumé fourni par la source

This Research Topic is a part of the Methods and Applications in Physiology series and builds on the successful publication of the "Methods and Applications in Vascular Physiology: 2022".In current issue, we included manuscripts devoted to novel methods and protocols and extension of existing ones covering wide aspects of the vascular physiology at the molecular, cellular, intact tissue, and systems levels.For instance, the articles from Wallis et al. and Schubert et al. present ex vivo results describing a novel methodology to assess NO production and discussing the relevance of the different models of myography on isolated blood vessels, respectively.On another hand, in vivo studies i) described the interaction between the cardio-ankle vascular index and blood pressure during haemodialysis (Sato et al.), ii) compared the merits of a large array of techniques used to assess aortic systolic and pulse pressure (Bia et al.) and iii) proposed a common outcome measure, namely, the vascular health index (VHI), developed to reconcile multiple measures routinely used to assess vascular function (Menon et al.).A more detailed overview of these manuscripts included as part of this Research Topic is provided below.The manuscript by Wallis et al. made a substantial contribution to the assessment of NO availability, and therefore vascular function, in intact arteries with fluorescent dyes.Accurate measurement of NO in arteries is problematic since this is an unstable and highly lipophilic free radical.The authors characterised the rhodamine-based DAR-4M AM and copper-based Cu2FL2E fluorescent NO-dyes, and refined their application in rat isolated resistance arteries in a pressure myography or en face imaging of endothelial cells.The comparison of both dies revealed that while they similarly responded to NO generated extracellularly from the NO donor SNAP, only DAR-4M responded to endogenous NO production and NO donors within intact arteries.Then, this study proposed a simple and reproducible approach based on the use of the fluorescent rhodamine-based NO dye, DAR-4M AM, for visualising NO production in isolated mesenteric arteries.This novel assay has the potential to be applied to other types of arteries to continuously monitor NO release,

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.