Renal function is a major predictor of circulating acyl-CoA-binding protein/diazepam-binding inhibitor

Le résumé fourni par la source

Objective Acyl-CoA-binding protein (ACBP)/diazepam-binding inhibitor has recently been introduced as an endocrine factor affecting food intake and lipid metabolism. ACBP is dysregulated in catabolic/malnutrition states like sepsis or systemic inflammation. However, regulation of ACBP has not been investigated in human and murine chronic kidney disease (CKD) and kidney failure (KF), so far. Design/Methods Serum ACBP levels were quantified by enzyme-linked immunosorbent assay (ELISA) in 60 subjects with KF on chronic hemodialysis compared to 60 subjects with an estimated glomerular filtration rate > 50 ml/min/1.73m 2 in a cross-sectional cohort. Furthermore, mRNA expression of mACBP was determined in two CKD mouse models compared to two different groups of non-CKD control mice. Moreover, mRNA expression of mACBP was investigated in cultured, differentiated mouse brown and white adipocytes after treatment with the uremic toxin indoxyl sulfate. Results Median [interquartile range] serum ACBP was significantly, almost 20-fold, increased in KF (514.0 [339.3] µg/l) compared to subjects with a preserved renal function (26.1 [39.1] µg/l) (p<0.001). Renal function was the most important, inverse predictor of circulating ACBP in multivariate analysis (standardized β=-0.839; p<0.001). Increased ACBP levels were not caused by augmented mACBP mRNA expression in different tissues of CKD mice in vivo or in indoxyl sulfate-treated adipocytes in vitro. Conclusions Circulating ACBP inversely associates with renal function, most likely through renal retention of the cytokine. Future studies need to investigate ACBP physiology in malnutrition-related disease states, such as CKD. Markers of renal function need to be reported in future studies on ACBP. Publication History Article published online: 02 May 2023 © 2023. Thieme. All rights reserved. Georg Thieme Verlag Rüdigerstraße 14, 70469 Stuttgart, Germany

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.