Oral Administration of Lacticaseibacillus rhamnosus CRL1505 Modulates Lung Innate Immune Response against Klebsiella pneumoniae ST25
Rattachement africain : ar, jp. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Orally administered Lacticaseibacillus rhamnosus CRL1505 enhances respiratory immunity, providing protection against respiratory viruses and Streptococcus pneumoniae. However, the capacity of the CRL1505 strain to improve respiratory immunity against Gram-negative bacterial infections has not been evaluated before. The aim of this work was to evaluate whether the Lcb. rhamnosus CRL1505 was able to beneficially regulate the respiratory innate immune response and enhance the resistance to hypermucoviscous KPC-2-producing Klebsiella pneumoniae of the sequence type 25 (ST25). BALB/c mice were treated with the CRL1505 strain via the oral route and then nasally challenged with K. pneumoniae ST25 strains LABACER 01 or LABACER 27. Bacterial cell counts, lung injuries and the respiratory and systemic innate immune responses were evaluated after the bacterial infection. The results showed that K. pneumoniae ST25 strains increased the levels of TNF-α, IL-1β, IL-6, IFN-γ, IL-17, KC and MPC-1 in the respiratory tract and blood, as well as the numbers of BAL neutrophils and macrophages. Mice treated with Lcb. rhamnosus CRL1505 had significantly lower K. pneumoniae counts in their lungs, as well as reduced levels of inflammatory cells, cytokines and chemokines in the respiratory tract and blood when compared to infected controls. Furthermore, higher levels of the regulatory cytokines IL-10 and IL-27 were found in the respiratory tract and blood of CRL1505-treated mice than controls. These results suggest that the ability of Lcb. rhamnosus CRL1505 to help with the control of detrimental inflammation in lungs during K. pneumoniae infection would be a key feature to improve the resistance to this pathogen. Although further mechanistic studies are necessary, Lcb. rhamnosus CRL1505 can be proposed as a candidate to improve patients’ protection against hypermucoviscous KPC-2-producing strains belonging to the ST25, which is endemic in the hospitals of our region.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Oral Administration of Lacticaseibacillus rhamnosus CRL1505 Modulates Lung Innate Immune Response against Klebsiella pneumoniae ST25
- Date Crossref
- 28/04/2023
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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National University of Tucumán pays non établi dans la noticeUniversité ou école supérieure
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Consejo Nacional de Investigaciones Científicas y Técnicas pays non établi dans la noticeOrganisme public
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Tohoku University pays non établi dans la noticeUniversité ou école supérieure
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Miyagi University Department of Food pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Biochemistry Laboratory of Antimicrobials pays non établi dans la noticeUniversité ou école supérieure
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Laboratory of Immunobiotechnology pays non établi dans la noticeStructure de recherche
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Graduate School of Agricultural Science Laboratory of Animal Food Function pays non établi dans la noticeUniversité ou école supérieure
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Graduate School of Pharmaceutical Sciences Laboratory of Molecular Genetics pays non établi dans la noticeUniversité ou école supérieure
National University of Tucumán, Consejo Nacional de Investigaciones Científicas y Técnicas et Tohoku University, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.