Genome‐wide analysis, metabolomics, and transcriptomics reveal the molecular basis of ZlRc overexpression in promoting phenolic compound accumulation in rice seeds
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Le résumé fourni par la source
Abstract Chinese wild rice (Zizania latifolia) is rich in phenolic compounds, particularly flavonoids. This study identified 203 basic helix‐loop‐helix (bHLHs) in Z. latifolia and showed that ZlbHLH196 (Zla16G011250) corresponds to the ZlRc gene in Z. latifolia, with its protein product localizing to the nucleus. Notably, the pericarps of ZlRc‐overexpressing (OE) rice are brown, whereas those of wild‐type (WT) rice are nonpigmented. The total phenolic, flavonoid, and proanthocyanidin contents, antioxidant activity, as well as enzyme inhibitory effects of ZlRc‐OE rice were significantly higher than those of WT rice. Overall, 221 differential phenolic metabolites were identified between ZlRc‐OE and WT rice, among which 198 were upregulated in the former. Additionally, a total of 227 differentially expressed genes were identified between ZlRc‐OE and WT rice, with 173 upregulated. Kyoto Encyclopedia of Genes and Genomes annotation and enrichment analysis of phenolic metabolites revealed enhanced isoflavonoid, flavone, flavonol, and flavonoid biosynthesis pathways in ZlRc‐OE rice, which, furthermore, showed a markedly upregulated expression and significantly higher activities of four key flavonoid biosynthesis–related enzymes (phenylalanine ammonia‐lyase, chalcone synthase, chalcone‐flavanone isomerase, and dihydroflavonol 4‐reductase). These findings show that ZlRc‐overexpression promotes phenolic compound accumulation in rice seeds and can be used to bioaugment rice phenolic content.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Genome‐wide analysis, metabolomics, and transcriptomics reveal the molecular basis of <i>ZlRc</i> overexpression in promoting phenolic compound accumulation in rice seeds
- Date Crossref
- 27/04/2023
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Tobacco Research Institute pays non établi dans la noticeStructure de recherche
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Chinese Academy of Agricultural Sciences pays non établi dans la noticeOrganisme public
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Agricultural Genomics Institute at Shenzhen pays non établi dans la noticeStructure de recherche
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Ministry of Agriculture and Rural Affairs pays non établi dans la noticeOrganisme public
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Agriculture and Agri-Food Canada pays non établi dans la noticeOrganisme public
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Guangdong Laboratory of Lingnan Modern Agriculture Shenzhen Branch pays non établi dans la noticeStructure de recherche
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Guelph Food Research Centre Agriculture and Agri‐Food Canada Guelph Ontario Canada pays non établi dans la noticeStructure de recherche
Tobacco Research Institute, Chinese Academy of Agricultural Sciences et Agricultural Genomics Institute at Shenzhen, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.