Data Supplement from Targeted Deletion and Lipidomic Analysis Identify Epithelial Cell COX-2 as a Major Driver of Chemically Induced Skin Cancer

Le résumé fourni par la source

Papillomas developed on Cox-2deltaE mice have a significantly increased percentage of small vessels and a significantly decreased percentage of (medium-sized + large-sized) vessels when compared to papillomas from littermate control Cox-2fl/fl mice. Vessel size analysis was performed with the Definiens' Tissue Studio program to determine vessel sizes from papillomas (n = 9) from Cox-2deltaE mice, papillomas (n = 19) from their littermate Cox-2fl/fl mice, papillomas (n = 9) from Cox-2deltaM mice, and papillomas (n = 10) from their Cox-2fl/fl littermate mice. Parameters were set to distinguish small vessels (<100µm2), medium-sized vessels (100-1000µm2), and large vessels (>1000µm2). The graph shows the vessel size distribution of small, medium-sized and large vessels in papillomas from each of the four cohorts. The percentage of small vessels in papillomas of Cox-2deltaE mice was significantly increased when compared to the percentage of small vessels in papillomas of their Cox-2fl/fl littermates (p = 0.003), and the percentage of (medium-sized + large vessels) was correspondingly reduced (p = 0.002). In contrast, vessel size distribution did not differ for papillomas from Cox-2deltaM mice and their Cox-2fl/fl littermates. Data were analyzed using an unpaired Student's t test. Error bars are SD. * indicates p<0.05.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.