Supplementary Methods, Figures 1 - 7 from Genetic Regulation of Fate Decisions in Therapeutic T Cells to Enhance Tumor Protection and Memory Formation

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Supplementary methods for flow cytometry and retroviral construct generation. Supplementary Figure 1. Expression of activated caspase-3 in CD8+ T cells overexpressing RHEB or PRAS40. Supplementary Figure 2. Expression of CD25 and CD69 in CD8+ T cells overexpressing RHEB or PRAS40. Supplementary Figure 3. Expression of killer cell lectin-like receptor subfamily G member 1 in CD8+ T cells overexpressing RHEB or PRAS40. Supplementary Figure 4. Recall immunity is impaired in CD8+ T cells overexpressing RHEB or PRAS40. Supplementary Figure 5. GFP induction with Doxycycline in CD8+ T cells transduced with iGFP and iPRAS vectors. Supplementary Figure 6. Levels of phosphorylated S6 after stimulation of CD8+ T cells transduced with pMP71-PRAS40 or pMP71-vector control. Supplementary Figure 7. Frequency of CD8+ T cells expressing shRNAs against mTOR or Raptor in peripheral blood 10 days after injection in tumor-bearing hosts.

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