Supplementary Data from A Novel l-Asparaginase with low l-Glutaminase Coactivity Is Highly Efficacious against Both T- and B-cell Acute Lymphoblastic Leukemias In Vivo

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Supplementary methods Supplementary Text: Wolinella succinogenes as a low L-glutaminase L-asparaginase Supplementary Figures: • Fig. S1. In vivo imaging showing that the L-glutaminase-diminished ErA-63Q and ErA-DM variants eliminate T-ALL LOUCY cells as effectively as the high L-glutaminase ErA-WT. • Fig. S2: In vivo imaging showing that the low L-glutaminase ErA-DM variant eliminates B-ALL SUP-B15 cells as effectively as the high L-glutaminase ErA-WT. • Fig. S3. In vivo imaging showing that the ultra-low L-glutaminase ErA-TM variant eliminates T-ALL LOUCY cells as effectively as the high L-glutaminase ErA-WT. • Fig. S4. In vitro sensitivity of two human ALL cell lines to L-asparaginases. • Fig. S5: Calibration between the in vivo imaging flux intensity and the measured %huCD45+ levels in peripheral blood (PB). • Fig. S6. In vivo imaging with signal-to-color range adjusted. • Fig. S7. Correlation between L-glutaminase activity and the reduction in mice activity. • Fig. S8. Correlation between L-glutaminase activity of the ErA variants and mice weight loss. • Fig. S9. ASNS qPCR data for the LOUCY cell line and 5 ALL patient samples. Data. Biostatistics on BLI and weight data.

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