Supplementary Figures S1-S11 from Combined Genome and Transcriptome Analysis of Single Disseminated Cancer Cells from Bone Marrow of Prostate Cancer Patients Reveals Unexpected Transcriptomes

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Supplementary Figures S1-S11. Supplementary Figure S1 - Workflow employed in this study. Supplementary Figure S2 - Photographs of cell lines stained with isotype antibodies. Supplementary Figure S3 - Box-plot representing BM cell numbers before and after immunomagnetic selection, number of screened cells and number of isolated EpCAM+ cells. Supplementary Figure S4 - Photographs of cell lines stained for EpCAM. Supplementary Figure S5 - Photographs and flowcytometric analysis of BM of control patient spiked with DU145 cells. Supplementary Figure S6 - Expression analysis of BM cell pools. Supplementary Figure S7 - QC of WTA and overview of epithelial transcript expression. Supplementary Figure S8 - Results of expression profiling of all samples. Supplementary Figure S9 - Analysis of WTA controls for HBA2 transcript. Supplementary Figure S10 - CGH profiles of analysed single cells. Supplementary Figure S11 - Establishing conditions for specific amplification of selected fragments by PCR and confirmation of identity of amplified fragments.

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