Figure S3 from A Genome-Wide CRISPR Screen Identifies Genes Critical for Resistance to FLT3 Inhibitor AC220

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Supplementary Figure 3. AC220 inhibits FLT3-ITD downstream signaling pathways and SPRY3 or GSK3 deletions reactivate RAS/ERK and Wnt signaling pathways in the presence of AC220 in Ba/F3 cells. (A) Ba/F3 and Ba/F3 FLT3-ITD cells were treated with FLT3-ligand or IL-3 and collected and lysed. Western blots analysis was carried out. (B) 0.3 X 106 cells were starved overnight and treated with DMSO or 10nM AC220 in combination with 3uM Chiron. The cells were harvested at indicated times and lysed. The western blots analysis was performed. (C) 0.3 X 106 MV4-11 cells were starved overnight and treated with FGF-1 (100ng/ml) and heparin (10ug/ml) in combination with 10 nM AC220. The cells were harvested at indicated times and lysed. Western blots analysis was performed. (D) 0.3 X 106 cells were starved overnight and treated with DMSO or 10nM AC220 in combination with 3uM Chiron. Western blots analysis was carried out.

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