Opening a window to the acutely injured brain: Simultaneous retinal and cerebral vascular monitoring in rats
Rattachement africain : de, ch. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Many recent research projects have described typical chronic changes in the retinal vasculature for diverse neurovascular and neurodegenerative disorders such as stroke or Alzheimer's disease. Unlike cerebral vasculature, retinal blood vessels can be assessed non-invasively by retinal vessel analysis. To date, there is only a little information about potential simultaneous reactions of retinal and cerebral vessels in acute neurovascular diseases. The field of applications of retinal assessment could significantly be widened if more information about potential correlations between those two vascular beds and the feasibility of non-invasive retinal vessel analysis in acute neurovascular disease were available. Here, we present our protocol for the simultaneous assessment of retinal and cerebral vessels in an acute setting in anesthetized rats using a non-invasive retinal vessel analyzer and a superficial tissue imaging system for laser speckle contrast analysis via a closed bone window. We describe the experimental set-up in detail, outline the pitfalls of repeated retinal vessel analyses in an experimental set-up of several hours, and address issues that arise from the simultaneous use of two different assessment tools. Finally, we demonstrate the robustness and variability of the reactivity of retinal vessels to hypercapnia at baseline as well as their reproducibility over time using two anesthetic protocols common for neurovascular research. In summary, the procedures described in this protocol allow us to directly compare retinal and cerebral vascular beds and help to substantiate the role of the retina as a “window to the brain.”
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Opening a window to the acutely injured brain: Simultaneous retinal and cerebral vascular monitoring in rats
- Date Crossref
- 24/03/2023
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
RWTH Aachen University pays non établi dans la noticeUniversité ou école supérieure
-
Fraunhofer Institute for Toxicology and Experimental Medicine pays non établi dans la noticeStructure de recherche
-
Kantonsspital Aarau pays non établi dans la noticeÉtablissement de santé
-
University of Cologne Institute for Neurophysiology pays non établi dans la noticeUniversité ou école supérieure
-
Medical Faculty Department of Neurosurgery pays non établi dans la noticeUniversité ou école supérieure
-
Department of Neurosurgery pays non établi dans la noticeInstitution
RWTH Aachen University, Fraunhofer Institute for Toxicology and Experimental Medicine et Kantonsspital Aarau, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.