Spherical Body Protein 4 from Babesia bigemina: A Novel Gene That Contains Conserved B-Cell Epitopes and Induces Cross-Reactive Neutralizing Antibodies in Babesia ovata
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Le résumé fourni par la source
Bovine babesiosis is a tick-transmitted disease caused by intraerythrocytic protozoan parasites of the genus Babesia. Its main causative agents in the Americas are Babesia bigemina and Babesia bovis, while Babesia ovata affects cattle in Asia. All Babesia species secrete proteins stored in organelles of the apical complex, which are involved in all steps of the invasion process of vertebrate host cells. Unlike other apicomplexans, which have dense granules, babesia parasites instead have large, round intracellular organelles called spherical bodies. Evidence suggests that proteins from these organelles are released during the process of invading red blood cells, where spherical body proteins (SBPs) play an important role in cytoskeleton reorganization. In this study, we characterized the gene that encodes SBP4 in B. bigemina. This gene is transcribed and expressed in the erythrocytic stages of B. bigemina. The sbp4 gene consists of 834 nucleotides without introns that encode a protein of 277 amino acids. In silico analysis predicted a signal peptide that is cleaved at residue 20, producing a 28.88-kDa protein. The presence of a signal peptide and the absence of transmembrane domains suggest that this protein is secreted. Importantly, when cattle were immunized with recombinant B. bigemina SBP4, antibodies identified B. bigemina and B. ovata merozoites according to confocal microscopy observations and were able to neutralize parasite multiplication in vitro for both species. Four peptides with predicted B-cell epitopes were identified to be conserved in 17 different isolates from six countries. Compared with the pre-immunization sera, antibodies against these conserved peptides reduced parasite invasion in vitro by 57%, 44%, 42%, and 38% for peptides 1, 2, 3, and 4, respectively (p < 0.05). Moreover, sera from cattle infected with B. bigemina cattle contained antibodies that recognized the individual peptides. All these results support the concept of spb4 as a new gene in B. bigemina that should be considered a candidate for a vaccine to control bovine babesiosis.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Spherical Body Protein 4 from Babesia bigemina: A Novel Gene That Contains Conserved B-Cell Epitopes and Induces Cross-Reactive Neutralizing Antibodies in Babesia ovata
- Date Crossref
- 22/03/2023
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Autonomous University of Queretaro pays non établi dans la noticeUniversité ou école supérieure
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Agricultural Research Service pays non établi dans la noticeOrganisme public
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United States Department of Agriculture pays non établi dans la noticeOrganisme public
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Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias pays non établi dans la noticeStructure de recherche
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Universidad Nacional Autónoma de México pays non établi dans la noticeUniversité ou école supérieure
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Obihiro University of Agriculture and Veterinary Medicine National Research Center for Protozoan Diseases pays non établi dans la noticeUniversité ou école supérieure
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Universidad Autónoma de la Ciudad de México pays non établi dans la noticeUniversité ou école supérieure
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C.A. Salud Animal y Microbiologia Ambiental pays non établi dans la noticeInstitution
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Immunology and Vaccines Laboratory pays non établi dans la noticeStructure de recherche
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College of Natural Sciences Ph.D. Program in Biological Sciences pays non établi dans la noticeUniversité ou école supérieure
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Animal Diseases Research Unit pays non établi dans la noticeStructure de recherche
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CENID-Salud Animal e Inocuidad/INIFAP pays non établi dans la noticeInstitution
Autonomous University of Queretaro, Agricultural Research Service et United States Department of Agriculture, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.