Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells
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Le résumé fourni par la source
Introduction: Three-dimensional (3D) bioprinting is a promising technique for the development of neuronal in vitro models because it controls the deposition of materials and cells. Finding a biomaterial that supports neural differentiation in vitro while ensuring compatibility with the technique of 3D bioprinting of a self-standing construct is a challenge. Methods: In this study, gelatin methacryloyl (GelMA), methacrylated alginate (AlgMA), and hyaluronic acid (HA) were examined by exploiting their biocompatibility and tunable mechanical properties to resemble the extracellular matrix (ECM) and to create a suitable material for printing neural progenitor cells (NPCs), supporting their long-term differentiation. NPCs were printed and differentiated for up to 15 days, and cell viability and neuronal differentiation markers were assessed throughout the culture. Results and Discussion: This composite biomaterial presented the desired physical properties to mimic the ECM of the brain with high water intake, low stiffness, and slow degradation while allowing the printing of defined structures. The viability rates were maintained at approximately 80% at all time points. However, the levels of β -III tubulin marker increased over time, demonstrating the compatibility of this biomaterial with neuronal cell culture and differentiation. Furthermore, these cells showed increased maturation with corresponding functional properties, which was also demonstrated by the formation of a neuronal network that was observed by recording spontaneous activity via Ca 2+ imaging.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells
- Date Crossref
- 03/03/2023
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Institute for Bioengineering of Catalonia Nanobioengineering Group pays non établi dans la noticeStructure de recherche
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Biomedical Research Networking Center in Bioengineering pays non établi dans la noticeInstitution
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Universitat de Barcelona pays non établi dans la noticeUniversité ou école supérieure
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Consorci Institut D'Investigacions Biomediques August Pi I Sunyer pays non établi dans la noticeStructure de recherche
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University of Barcelona Department of Electronic and Biomedical Engineering pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Medicine and Health Sciences Creatio - Production and Validation Center of Advanced Therapies pays non établi dans la noticeUniversité ou école supérieure
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Research Foundation Clinic Barcelona-August Pi i Sunyer Biomedical Research Institute (FRCB-IDIBAPS) pays non établi dans la noticeStructure de recherche
Nanobioengineering Group — Institute for Bioengineering of Catalonia, Biomedical Research Networking Center in Bioengineering et Universitat de Barcelona, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.