Oropouche virus as an emerging cause of acute febrile illness in Colombia
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Le résumé fourni par la source
Arbovirus infections are frequent causes of acute febrile illness (AFI) in tropical countries. We conducted health facility-based AFI surveillance at four sites in Colombia (Cucuta, Cali, Villavicencio, Leticia) during 2019-2022. Demographic, clinical and risk factor data were collected from persons with AFI that consented to participate in the study (n = 2,967). Serologic specimens were obtained and tested for multiple pathogens by RT–PCR and rapid test (Antigen/IgM), with 20.7% identified as dengue positive from combined testing. Oropouche virus (OROV) was initially detected in serum by metagenomic next-generation sequencing (mNGS) and virus target capture in a patient from Cúcuta. Three additional infections from Leticia were confirmed by conventional PCR, sequenced, and isolated in tissue culture. Phylogenetic analysis determined there have been at least two independent OROV introductions into Colombia. To assess OROV spread, a RT-qPCR dual-target assay was developed which identified 87/791 (10.9%) viremic cases in AFI specimens from Cali (3/53), Cucuta (3/19), Villavicencio (38/566), and Leticia (43/153). In parallel, an automated anti-nucleocapsid antibody assay detected IgM in 27/503 (5.4%) and IgG in 92/568 (16.2%) patients screened, for which 24/68 (35.3%) of PCR positives had antibodies. Dengue was found primarily in people aged <18 years and linked to several clinical manifestations (weakness, skin rash and petechiae), whereas Oropouche cases were associated with the location, climate phase, and odynophagia symptom. Our results confirm OROV as an emerging pathogen and recommend increased surveillance to determine its burden as a cause of AFI in Colombia.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Oropouche virus as an emerging cause of acute febrile illness in Colombia
- Date Crossref
- 08/12/2022
- Éditeur
- Informa UK Limited
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Universidad Nacional de Colombia pays non établi dans la noticeUniversité ou école supérieure
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Abbott (United States) pays non établi dans la noticeEntreprise
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Bioversity International pays non établi dans la noticeOrganisation à but non lucratif
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Abbott (Germany) pays non établi dans la noticeEntreprise
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Abbott Fund pays non établi dans la noticeOrganisation à but non lucratif
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University of Wisconsin–Madison Global Health Institute pays non établi dans la noticeUniversité ou école supérieure
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University of Wisconsin Health pays non établi dans la noticeÉtablissement de santé
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Abbott Pandemic Defense Coalition pays non établi dans la noticeInstitution
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Global Health Institute One-Health Colombia pays non établi dans la noticeStructure de recherche
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Abbott Diagnostics pays non établi dans la noticeInstitution
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Infectious Diseases Research pays non établi dans la noticeInstitution
Universidad Nacional de Colombia, Abbott (United States) et Bioversity International, avec 8 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.