Assessing the Potential of Untargeted SWATH Mass Spectrometry-Based Metabolomics to Differentiate Closely Related Exposures in Observational Studies
Rattachement africain : ch, nl, ca. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Mass spectrometry (MS) is increasingly used in clinical studies to obtain molecular evidence of chemical exposures, such as tobacco smoke, alcohol, and drugs. This evidence can help verify clinical data retrieved through anamnesis or questionnaires and may provide insights into unreported exposures, for example those classified as the same despite small but possibly relevant chemical differences or due to contaminants in reported exposure compounds. Here, we aimed to explore the potential of untargeted SWATH metabolomics to differentiate such closely related exposures. This data-independent acquisition MS-based profiling technique was applied to urine samples of 316 liver and 570 kidney transplant recipients from the TransplantLines Biobank and Cohort Study (NCT03272841), where we focused on the immunosuppressive drug mycophenolate, which is either supplied as a morpholino-ester prodrug or as an enteric-coated product, the illicit drug cocaine, which is usually supplied as an adulterated product, and the proton pump inhibitors omeprazole and esomeprazole. Based on these examples, we found that untargeted SWATH metabolomics has considerable potential to identify different (unreported) exposure or co-exposure metabolites and may determine variations in their abundances. We also found that these signals alone may sometimes be unable to distinguish closely related exposures, and enhancement of differentiation, for example by integration with pharmacogenomics data, is needed.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Assessing the Potential of Untargeted SWATH Mass Spectrometry-Based Metabolomics to Differentiate Closely Related Exposures in Observational Studies
- Date Crossref
- 04/10/2022
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Geneva Department of Inorganic and Analytical Chemistry pays non établi dans la noticeUniversité ou école supérieure
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University Medical Center Groningen Department of Clinical Pharmacy and Pharmacology pays non établi dans la noticeÉtablissement de santé
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University of Groningen Unit of PharmacoTherapy pays non établi dans la noticeUniversité ou école supérieure
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Canadian Rheumatology Association pays non établi dans la noticeInstitution
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Ron Bonner Consulting pays non établi dans la noticeInstitution
Department of Inorganic and Analytical Chemistry — University of Geneva, Department of Clinical Pharmacy and Pharmacology — University Medical Center Groningen et Unit of PharmacoTherapy — University of Groningen, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.